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TranscriptomicsGene expression profiles of the diabetic glomerular endothelial cell
The objective of this study is to create an encyclopedia of all genes expressed in the glomerular endothelial cell under normal and diabetic conditions. We utilized Tie2-GFP transgenic mice to mark cells of the glomerular endothelium. To induce diabetic nephropathy (DB), a genetic model of DB, BKS...
ORGANISM(S): Mus musculus 
2010-08-16 | E-GEOD-21324 | biostudies-arrayexpress
TranscriptomicsGene expression profiles of the adult endothelial cell (GUDMAP Series id: 35)
The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The combination of fluorescent activated cell sorting (FACS) plus microarray analysis offers a powerful, efficient and e...
ORGANISM(S): Mus musculus 
2010-01-25 | E-GEOD-20004 | biostudies-arrayexpress
TranscriptomicsGene expression profiles of E15.5 endothelial cells (GUDMAP Series_id: 38)
The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The combination of fluorescent activated cell sorting (FACS) plus microarray analysis offers a powerful, efficient and e...
ORGANISM(S): Mus musculus 
2010-03-22 | E-GEOD-20991 | biostudies-arrayexpress
TranscriptomicsRNA-SEQ analysis of single cells extracted from the metanephric mesenchyme of Embryonic day 11.5 (E11.5) Crym-EGFP transgenic mice.
We used micro-dissection with FACS sorting techniques to isolate single cells from the metanephric mesenchyme of the E11.5 developing kidney. A subset of these single cell populations is analysed individually via Fluidigm single cell analysis. This analysis will determine the transcriptional profi...
ORGANISM(S): Mus musculus 
2014-08-01 | E-GEOD-59129 | biostudies-arrayexpress
TranscriptomicsRNA-SEQ analysis of single cells extracted from the renal vesicles of Crym-EGFP transgenic post natal day 4 (P4) mice.
We used micro-dissection with FACS sorting techniques to isolate renal vesicle single cell types from post natal (P4) kidneys. A subset of these single cell populations is analysed individually via Fluidigm single cell analysis. This analysis will determine the transcriptional profile of each cell...
ORGANISM(S): Mus musculus 
2014-08-01 | E-GEOD-59130 | biostudies-arrayexpress
TranscriptomicsRNA-SEQ analysis of single cells extracted from E12.5 embryonic kidneys.
We used micro-dissection and trypsinization techniques to isolate single cells from the E12.5 total kidney. A subset of these single cell populations is analysed individually via Fluidigm single cell analysis. This analysis will determine the transcriptional profile of each cell type, identify com...
ORGANISM(S): Mus musculus 
2014-08-01 | E-GEOD-59127 | biostudies-arrayexpress
TranscriptomicsNotch1 dose modulates endothelial gene expression
Purpose: To use RNA-Seq analysis of endothelial cell in various Notch1 alllels in order to determine transcrptional differencesas a consequence of Notch dose. Methods: Using a FACS sorting we generated high-throughput RNA-SEQ data of endothelials in various Notch1 alleles during development Results...
ORGANISM(S): Mus musculus 
2015-08-04 | E-GEOD-69276 | biostudies-arrayexpress
TranscriptomicsGene expression profiles of components isolated from developing kidney at E11.5, E12.5 & E15.5 using LCM. (GUDMAP Series ID: 10)
The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The combination of laser capture microdissection (LCM) plus microarray analysis offers a powerful, efficient and effecti...
ORGANISM(S): Mus musculus 
2006-11-18 | E-GEOD-6290 | biostudies-arrayexpress
TranscriptomicsPathogenic pathways are activated in each major cell type of the glomerulus in the Cd2ap mutant mouse model of focal segmental glomerulosclerosis
Mutations in several genes expressed in podocytes, including Cd2ap, have been associated with focal segmental glomerulosclerosis in humans. Mutant mouse models provide an opportunity to better understand the molecular pathology that drives these diseases. In this report we use a battery of transgeni...
ORGANISM(S): Mus musculus 
2015-03-03 | E-GEOD-63272 | biostudies-arrayexpress
TranscriptomicsGene expression profiles of the adult capsular cell (GUDMAP Series_id: 36)
The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The combination of fluorescent activated cell sorting (FACS) plus microarray analysis offers a powerful, efficient and e...
ORGANISM(S): Mus musculus 
2010-03-05 | E-GEOD-20639 | biostudies-arrayexpress
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