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TranscriptomicsIn situ-synthesized novel microarray optimized for mouse stem cell and early developmental expression profiling: Part2
E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples and compared on a novel microarray design to validate the system. A "self-versus-self" experimental design was u...
ORGANISM(S): Mus musculus 
2010-06-30 | E-GEOD-3513 | biostudies-arrayexpress
TranscriptomicsIn situ-synthesized novel microarray optimized for mouse stem cell and early developmental expression profiling: Part1
E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples and compared on a novel microarray design to validate the system. A "dye-swapped" experimental design was used t...
ORGANISM(S): Mus musculus 
2010-06-30 | E-GEOD-3516 | biostudies-arrayexpress
TranscriptomicsIn situ-synthesized novel microarray optimized for mouse stem cell and early developmental expression profiling: Part 3
E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples at various input levels. A "full-scale" reference was prepared using the standard input level of 6.0 ug total RN...
ORGANISM(S): Mus musculus 
2010-06-30 | E-GEOD-3507 | biostudies-arrayexpress
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