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TranscriptomicsRNA-Seq of monoclonal cell lines after CRISPR/Cas9-mediated editing of box C/D snoRNA genes (SNORDs)
In this study monoclonal cell lines carrying mutations in snoRNA genes (SNORD74, SNORD75, SNORD77, SNORD80) were obtained. Next, whole transcriptome analysis of obtained cell lines was performed on an Illumina NextSeq 500 platform. RNA-seq libraries was constructed on PolyA mRNA-enriched fraction. T...
ORGANISM(S): Homo sapiens 
2019-10-14 | E-MTAB-8269 | biostudies-arrayexpress
TranscriptomicsNext generation sequencing identifies discrete classes of box C/D snoRNAs featuring different ends and RNA binding protein dependency
The paired-end next-generation sequencing of all small RNAs of less than 200 nucleotides in length from four different human cell lines (SKOV3ip1, MCF-7, BJ-Tielf, INOF) allowed us to determine the exact sequence(s) and variations of human box C/D snoRNAs (small nucleolar RNAs), revealing processing...
ORGANISM(S): Homo sapiens 
2014-07-01 | E-GEOD-55946 | biostudies-arrayexpress
ProteomicsEnhanced snoMEN vectors facilitate establishment of GFP–HIF-1? protein replacement human cell lines
The snoMEN (snoRNA Modulator of gene ExpressioN) vector technology was developed from a human box C/D snoRNA, HBII-180C, which contains an internal sequence that can be manipulated to make it complementary to RNA targets, allowing knock-down of targeted genes. Here we have screened additional human ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-31 | MSV000080828 | MassIVE
GenomicsThe telomere-binding protein Tbf1 demarcates snoRNA gene promoters in Saccharomyces cerevisiae
The majority of Saccharomyces cerevisiae snoRNA promoters contain an aRCCCTaa sequence motif located at the upstream border of a TATA-containing nucleosome-free region. Genome-wide ChIP-seq analysis showed that these motifs are bound in vivo by Tbf1, a telomere-binding protein known to recognize mam...
ORGANISM(S): Saccharomyces cerevisiae 
2010-05-28 | E-GEOD-20870 | biostudies-arrayexpress
GenomicsThe human box C/D snoRNA U3 is a miRNA source
The human box C/D snoRNA U3 is a miRNA source
PRJNA563562 | ENA
ProteomicsIMPAIRED CHONDROCYTE U3 SNORNA EXPRESSION IN OSTEOARTHRITIS AND ITS IMPACT ON THE CHONDROCYTE’S PROTEIN TRANSLATION APARATUS
Osteoarthritis (OA) is a chronic debilitating joint disease which is strongly associated with ageing. OA involves pathological cellular processes in all joint structures and affects articular cartilage integrity, leading to dysfunctional joint articulation. The biomolecular processes that catalyze t...
ORGANISM(S): Homo sapiens (Human) 
2020-08-21 | PXD017253 | Pride
Osteoarthritis Snorna Translation 
GenomicsThe snoGloBe interaction predictor reveals a broad spectrum of C/D snoRNA RNA targets
The snoGloBe interaction predictor reveals a broad spectrum of C/D snoRNA RNA targets
PRJNA763506 | ENA
TranscriptomicsHuman PA-1 cells treated with scrambled or specific ASOs
We describe the discovery of sno-lncRNAs, a class of nuclear-enriched intron-derived long noncoding RNAs (lncRNAs) that are processed on both ends by the snoRNA machinery. During exonucleolytic trimming, the sequences between the snoRNAs are not degraded, leading to the accumulation of lncRNAs flank...
ORGANISM(S): Homo sapiens 
2013-04-26 | E-GEOD-38541 | biostudies-arrayexpress
TranscriptomicsHuman Nonsense-Mediated RNA Decay Initiates Widely by Endonucleolysis and Targets snoRNA Host Genes
Eukaryotic RNAs with premature termination codons (PTCs) are eliminated by nonsense-mediated decay (NMD). While human nonsense RNA degradation can be initiated either by an endonucleolytic cleavage event near the PTC or through decapping, the individual contribution of these activities on endogenous...
ORGANISM(S): Homo sapiens 
2014-11-18 | E-GEOD-57433 | biostudies-arrayexpress
GenomicsThe human box C/D snoRNA U3 is a miRNA source
small RNA seq form Hek 293 using IonTorrent Sequencing; To assess the general expression of snoRNA derived miRNA like fragments in Hek293. Three independent small RNA libraries were sequenced, aligned and subset for snoRNA (C/D, H/ACA, Small Cajal bodies); analysis of read-length for individual snoR...
ORGANISM(S): Homo sapiens 
2019-09-04 | GSE136745 | GEO
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