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Drastic alteration in macronutrients causes large changes in gene expression in the photosynthetic unicellular alga Chlamydomonas reinhardtii. Preliminary data suggested that cells follow a biphasic response to this change hinging on the initiation of lipid accumulation, and we hypothesized that ...

2015-06-29 | MTBLS208 | MetaboLights

In the actual context of climate changing environments, photosynthetic organisms need to adapt to more extreme conditions. Microalgae can be excellent organisms to understand molecular mechanisms that activate survival mechanisms under stress. Chlamydomonas reinhardtii signaling mutants are extre...

2024-12-05 | MTBLS10779 | MetaboLights
Collection of various small RNA libraries of C. Reinhardtii sequenced using Illumina sRNA sequencing. Libraries are derived from various C. Reinhardtii genotypes (CC1883, CC4350, CC125, J), ecotypes, life cycles (Vegatative, Zygote) treatments and mutants. Preparation was done over a period of sev...
ORGANISM(S): Chlamydomonas reinhardtii 
Microalgae are photosynthetic organisms capable of autotrophic growth. Their applicability in multiple industrial fields has been largely studied, thanks to their ability to fixate CO2 into high added value organic products like fatty acids and carotenoids. However, our under-standing of the cellula...
2026-06-09 | MTBLS14720 | MetaboLights
Biological rhythms in response to both endogenous (circadian) and exogenous (e.g. diel) cycles, with a period of ~24 hours, are a prominent feature of many living systems. In green algal species, knowledge on the extent of diel rhythmicity of genome wide gene expression, its evolution, and its cis-r...
ORGANISM(S): Chlamydomonas reinhardtii 
This study aims at investigating the link between internalized inorganic or methyl Hg and the global expression of genes, obtained by high-throughput sequencing (RNA-Seq), in the microalga Chlamydomonas reinhardtii. Algal cells were exposed two hours in a simplified artificial medium spiked with ser...
ORGANISM(S): Chlamydomonas reinhardtii 
Chlamydomonas reinhardtii forms lipid droplets rich in triacylglycerols when nutrient-deprived. To begin studying the mechanisms underlying this process, N-deprivation was used to induce triacylglycerol accumulation and changes in developmental programs such as gametogenesis. Comparative global anal...
ORGANISM(S): Chlamydomonas reinhardtii 
We analysed global gene expression changes in Chlamydomonas reinhardtii in response to 1h UV-B, applied at the same low level that was seen to promote subsequent UV-B stress tolerance, in order to elucidate the transcriptional reprogramming that leads to UV-B acclimation. mRNA profiles generated by ...
ORGANISM(S): Chlamydomonas reinhardtii 
Algal photo-bio hydrogen production, a promising method for producing clean and renewable fuel in the form of hydrogen gas, has been studied extensively over the last few decades. In this study, microarray analyses were used to obtain a global expression profile of mRNA abundance in the green alga ...
ORGANISM(S): Chlamydomonas reinhardtii 
DCL3 is the main processor of miRNA precursors in Chlamydomonas reinhardtii. Because of that, and in order identify mRNA targets of miRNA-guided slicer complexes, we used RNAseq of the transcriptome in dcl3 mutant and parental lines.
ORGANISM(S): Chlamydomonas reinhardtii 
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