C57bl/6n - OmicsDI

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Single-Cell Extraction: For enzymatic digestion, pronase (Septomyces argeus, 1 mg/mL) was applied for 25 minutes at room temperature for P0 to P8 datasets. Cells were dissociated and triturated into a single-cell suspension in ACSF supplemented with 1% fetal calf serum (FCS) and DNase (1 µL/10 mL). Trituration was performed using three glass Pasteur pipettes of decreasing diameters. For P30 datasets, enzymatic digestion and cell dissociation were carried out using the Worthington Papain Dissociation System, following the manufacturer’s instructions. Single-Nuclei Extraction: For nuclei isolation, the Hibernate™-E Medium was removed, and 500 µL of chilled 0.1X NP40 Lysis Buffer was added. Tissue was homogenized using a Pellet Pestle (15 strokes) and incubated on ice for 5 minutes. The suspension was pipette-mixed 10 times using a wide-bore pipette tip and incubated for an additional 10 minutes on ice. Following lysis, 500 µL of chilled wash buffer was added, and the mixture was pipette-mixed 5 times using a regular-bore pipette tip. The suspension was filtered through a 30 µm cell strainer into a 50 mL tube to remove debris. The filtered suspension was transferred to a 1.5 mL tube and centrifuged at 950 × g for 10 minutes at 4°C. The supernatant was carefully removed, and the nuclei pellet was retained for downstream analysis. (2)
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CHEBI ID

CHEBI:16856 (4)
CHEBI:15741 (4)
CHEBI:18012 (4)
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CHEBI:17992 (3)
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Transcription profiling by array (38)
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Lab affiliation

DIVAS, Department of Veterinary Medicine and Animal Science (1)
King's BHF Centre of Research Excellence, King's College London, London, UK (1)
Institute of Experimental Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Martinistr. 52, 20246 Hamburg, Germany (1)
Molecular Proteomics Laboratory (1)
Proteomics Group Max Planck Institute of Experimental Medicine Hermann-Rein-Str. 3 37075 Goettingen, Germany Tel: +49-551-3899313 Fax: +49-551-3899323 (1)
Max Planck Institute for Biology of Ageing, Joseph-Stelzmann-Str. 9b, D-50931 Cologne, Germany Institute of Healthy Ageing, Department of Genetics, Evolution and Environment,University College London, Darwin Building, Gower Street, London WC1E 6BT, UK (1)
Helmholtz-Centre for Environmental Research – UFZ, Department of Molecular Toxicology (1)
Max Planck Institute for Biology of Ageing, Germany (1)
Boston University School of Medicine (1)
Max-Delbrück Center for Molecular Medicine (MDC) Robert-Rössle-Str. 10, 13125 Berlin, Germany (1)
Stomatological Hospital of Chongqing Medical University (1)
Department of Endocrinology, Tongji Hospital Affiliated to Tongji University, School of Medicine, Tongji University, 1239 Siping Road, Shanghai 200092, China. (1)
Institute of Physiology II, University of Münster, Münster, Germany (1)
DIVAS department of veterinary medicine and animal science (1)
Department of Analytical Chemistry, Faculty of Chemistry, University of Vienna (1)
Wellcome Trust Sanger Institute (1)

Tags

xref:PubMed:37564700 (7)
xref:PubMed:38684889 (5)
xref:PubMed:35645339 (4)
xref:PubMed:32234480 (4)
xref:PubMed:37467962 (3)
xref:PubMed:27309802 (3)
xref:PubMed:41099430 (3)
xref:PubMed:34558834 (3)
xref:PubMed:39875968 (3)
xref:PubMed:30606613 (3)
xref:PubMed:33232972 (2)
xref:PubMed:29973619 (2)
xref:PubMed:35722589 (2)
xref:PubMed:35709278 (2)
xref:PubMed:37020391 (2)
xref:PubMed:41275495 (2)
xref:PubMed:31152140 (2)
xref:PubMed:37769257 (2)
xref:PubMed:42467615 (2)
xref:PubMed:39173022 (2)
xref:PubMed:22952760 (2)
xref:PubMed:35915220 (2)
xref:PubMed:40340422 (2)
xref:PubMed:39904338 (2)
xref:PubMed:39048795 (2)
xref:PubMed:40709473 (2)
xref:ArrayExpress:E-ERAD-401 (1)
xref:ArrayExpress:E-ERAD-352 (1)
xref:PubMed:25557080 (1)
xref:ArrayExpress:E-ERAD-480 (1)

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Mus musculus strain:C57BL/6N | breed:C57BL/6N

Mus musculus strain:C57BL/6N | breed:C57BL/6N Raw sequence reads

PRJNA1515424 | ENA

Characterisation of adult C57BL/6N sperm small RNA

This study aimed at generating a reference data set of C57BL/6N small RNA expression at baseline conditions

ORGANISM(S): Mus musculus

2020-01-09 | E-MTAB-7617 | biostudies-arrayexpress

Mus musculus strain:C57BL/6N

Mus musculus strain:C57BL/6N Epigenomics

PRJNA1256669 | ENA

Characterisation of adult C57BL/6N sperm small RNA

Characterisation of adult C57BL/6N sperm small RNA

PRJEB30920 | ENA

Mus musculus strain:C57BL/6N

Mus musculus strain:C57BL/6N Raw sequence reads

PRJNA1359572 | ENA

Mus musculus strain:C57Bl/6N

Mus musculus strain:C57Bl/6N Raw sequence reads

PRJNA779821 | ENA

Mus musculus strain:C57Bl/6N

Mus musculus strain:C57Bl/6N Raw sequence reads

PRJNA436964 | ENA

Mus musculus strain:C57BL/6N

Mus musculus strain:C57BL/6N Raw sequence reads

PRJNA697862 | ENA

Tunicamycin induced ER stress in liver of C57Bl/6N mice

Tunicamycin induced ER stress in liver of C57Bl/6N mice

PRJNA704105 | ENA

Gene expression profiling in left cardiac ventricles of C57Bl/6J and C57Bl/6N male mice treated for 48 h with angiotensin II 500 ng/kg/min.

Analysis of angiotensin II effect on left ventricle at gene expression level. The hypothesis tested in the present study was that angiotensin II treatment may affect gene expression in left ventricle in a strain specific manner. Results provide important information about which genes respond to angi...

ORGANISM(S): Mus musculus

2014-12-12 | E-GEOD-53269 | biostudies-arrayexpress

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