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TranscriptomicsBSO-treated blastocysts vs CTL-treated blastocysts
In order to understand how in vitro culture affects embryonic quality, we analyzed survival and global gene expression in bovine blastocysts after exposure to increased oxidative stress conditions of IVC. A pro-oxidant agents that act intra-cellularly by inhibiting GSH synthesis (0.4 mM buthionine s...
ORGANISM(S): Bos taurus 
2012-11-14 | E-GEOD-42280 | biostudies-arrayexpress
TranscriptomicsAAPH-treated blastocysts vs CTL-treated blastocysts
In order to understand how in vitro culture affects embryonic quality, we analyzed survival and global gene expression in bovine blastocysts after exposure to increased oxidative stress conditions of IVC. A pro-oxidant agents that act extra-cellularly by promoting ROS production (0.01 mM 2,2'-azobis...
ORGANISM(S): Bos taurus 
2012-11-14 | E-GEOD-42279 | biostudies-arrayexpress
TranscriptomicsHigh Glucose (HG) treated morula vs control glucose (CTL) treated morula
Early bovine cleavage (day 0 to 3) was treated with high glucose (5mM) or control glucose (0.2mM) then total day 3 embryos were cultured in control condition until day 5 when gene expression was assessed in developping morula by microarray 4 replicates of 10 morulae/rep was produced for high glucose...
ORGANISM(S): Bos taurus 
2012-09-01 | E-GEOD-40521 | biostudies-arrayexpress
TranscriptomicsTranscriptomic signature in bovine blastocysts resulting from oxidative stress exposure at the time of embryonic genome activation
This SuperSeries is composed of the following subset Series: GSE42279: AAPH-treated blastocysts vs CTL-treated blastocysts GSE42280: BSO-treated blastocysts vs CTL-treated blastocysts Refer to individual Series
ORGANISM(S): Bos taurus 
2012-11-14 | E-GEOD-42281 | biostudies-arrayexpress
TranscriptomicsThe impact of exposure to serum lipids during in vitro culture on the transcriptome of bovine blastocysts
EtOH-Lipid form serum have been added in BSA-supplemented SOF during post-compaction development of IVP bovine embryos. Lipid-treated blastocysts were compared with control treated blastocysts (no lipid addition to BSA, EtOH balanced). Amplified anti-sense RNA from each sample (4 pools of control an...
ORGANISM(S): Bos taurus 
2014-01-09 | E-GEOD-53905 | biostudies-arrayexpress
TranscriptomicsMitochondrial supplementation at fertilization enhances early developmental outcome
Competent oocytes can be discriminated by BCB staining. Positive stained oocytes are considered more competent than BCB negative oocyte, and injection of BCB+ oocyte extracted mitochondria into BCB negative oocytse can increase fertilisation and blastocyst rate. Here we have analysed the impact of m...
ORGANISM(S): Sus scrofa 
2016-09-01 | E-GEOD-65310 | biostudies-arrayexpress
TranscriptomicsDifferential Gene Expression Profile in Bovine Blastocysts Resulting from Hyperglycemia Exposure during Early Cleavage Stages
IVP blastocysts exposed to hyperglycaemia during early phase of development, i.e from zygote to 8-16 cells stages. Genes expression analysis is done at day 7.5 (7 days of embryo culture). Total RNA form glucose treated blastocysts (4 replicates of 10 embryos each) and control treated blastocysts (4 ...
ORGANISM(S): Bos taurus 
2011-10-19 | E-GEOD-33008 | biostudies-arrayexpress
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