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MetabolomicsFlow cytometry has a significant impact on the cellular metabolome (CE-MS assay)
The characterization of specialized cell subpopulations in a heterogeneous tissue is essential for understanding organ function in health and disease. A popular method of cell isolation is fluorescence-activated cell sorting (FACS) based on probes that bind surface or intracellular markers. In this ...
2018-11-06 | MTBLS633 | MetaboLights
MetabolomicsFlow cytometry has a significant impact on the cellular metabolome (GC-MS assay)
The characterization of specialized cell subpopulations in a heterogeneous tissue is essential for understanding organ function in health and disease. A popular method of cell isolation is fluorescence-activated cell sorting (FACS) based on probes that bind surface or intracellular markers. In this ...
2018-11-06 | MTBLS634 | MetaboLights
MetabolomicsFlow cytometry has a significant impact on the cellular metabolome (General LC-MS assay)
The characterization of specialized cell subpopulations in a heterogeneous tissue is essential for understanding organ function in health and disease. A popular method of cell isolation is fluorescence-activated cell sorting (FACS) based on probes that bind surface or intracellular markers. In this ...
2018-11-06 | MTBLS629 | MetaboLights
MetabolomicsFlow cytometry has a significant impact on the cellular metabolome (Lipidomic LC-MS assay)
The characterization of specialized cell subpopulations in a heterogeneous tissue is essential for understanding organ function in health and disease. A popular method of cell isolation is fluorescence-activated cell sorting (FACS) based on probes that bind surface or intracellular markers. In this ...
2018-11-06 | MTBLS631 | MetaboLights
Metabolomicsthe intracellular flow of hexadecane in J31 cells

To investigate the metabolism and bioconversion of hexadecane in J31 cells, 13C-labeled hexadecane was added to the OM medium, and the intracellular flow of hexadecane in J31 cells was analyzed using the stable isotope technique via non-target metabolomics. study abstract/description

2025-11-04 | MTBLS13250 | MetaboLights
MetabolomicsHigh-performance nano-flow liquid chromatography column combined with high- and low-collision energy data-independent acquisition enables targeted and discovery identification of modified ribonucleotides by mass spectrometry (Data-independent acquisition)

Over 170 post-transcriptional RNA modifications have been described and are common in all kingdoms of life. These modifications range from methylation to complex chemical structures, with methylation being the most abundant. RNA modifications play a key role in RNA folding and function and their ...

2023-10-26 | MTBLS2959 | MetaboLights
ProteomicsOne-dimensional capillary liquid chromatographic separation coupled with tandem mass spectrometry unveils the Escherichia coli proteome on a microarray scale.
We successfully identified the proteome expressed in Escherichia coli (E. coli) cells on a microarray scale using one-dimensional capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) with a 350 cm long, 100 microm i. d., monolithic silica-C(18) capillary column. E. coli tryptic digest...
ORGANISM(S): Escherichia Coli 
2016-07-20 | PXD004605 | JPOST Repository
MetabolomicsHigh-performance nano-flow liquid chromatography column combined with high- and low-collision energy data-independent acquisition enables targeted and discovery identification of modified ribonucleotides by mass spectrometry (Mixed-mode column chromatography)

Over 170 post-transcriptional RNA modifications have been described and are common in all kingdoms of life. These modifications range from methylation to complex chemical structures, with methylation being the most abundant. RNA modifications play a key role in RNA folding and function and their ...

2023-10-26 | MTBLS2497 | MetaboLights
MetabolomicsCE-MS Metabolomic and LC-MS Proteomic Analyses of Breast Cancer Exosomes Reveal Alterations in Purine and Carnitine Metabolism

A nano sheath-flow capillary electrophoresis mass spectrometry (CE-MS) system with electrospray ionization was used to profile cationic metabolite cargo in exosomes secreted by non-tumorigenic MCF-10A and tumorigenic MDA-MB-231 breast epithelial cells. CE-MS-based metabolomics revealed increased ...

2024-11-14 | MTBLS11190 | MetaboLights
ProteomicsA simple and efficient approach for preparing cationic coating with tunable electroosmotic flow for capillary zone electrophoresis-mass spectrometry-based top-down proteomics
Capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) has become a valuable analytical technique in top-down proteomics (TDP) in recent years. CZE-MS/MS-based TDP typically employs separation capillaries with neutral coatings (i.e., linear polyacrylamide, LPA). However, issues related ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-10-15 | PXD051862 | Pride
Top-down proteomics  proteoform  capillary zone electrophoresis-mass spectrometry  covalent cationic coating  reproducibility  large proteoforms 
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