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TranscriptomicsTranscription profiling of E12.5 mouse whole embryo and E12.5 placenta total RNA pooled to create 25:75, 50:50, and 75:25 ratio mixtures to estimate transcript copy number
E12.5 mouse whole embryo and E12.5 placenta total RNA were pooled to create 25:75, 50:50, and 75:25 ratio mixtures, based on Bioanalyzer quantitation. These samples, along with the original unmixed RNAs, were used as templates for duplicate linear amplification labeling reactions. cRNA target mixtu...
ORGANISM(S): Mus musculus 
2005-01-01 | E-MNIA-67 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of E12.5 mouse whole embryo, E12.5 placenta, embryonic stem (ES) cells, and trophoblast stem (TS) cells on a novel microarray design to validate the system
E12.5 mouse whole embryo, E12.5 placenta, embryonic stem (ES) cells, and trophoblast stem (TS) cells were compared on a novel microarray design to validate the system. The in situ-synthesized 60-mer oligonucleotide microarray platform contains approximately 44,000 DNA features, and was designed to ...
ORGANISM(S): Mus musculus 
2005-01-01 | E-MNIA-66 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of E12.5 mouse whole embryos and placentas pooled within 3 litters to validate a new array
E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples and compared on a novel microarray design to validate the system. A "dye-swapped" experimental design was used t...
ORGANISM(S): Mus musculus 
2005-01-01 | E-MNIA-69 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of E12.5 mouse whole embryos and placentas pooled within 3 litters and used to valdiate a new array
E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples and compared on a novel microarray design to validate the system. A "self-versus-self" experimental design was u...
ORGANISM(S): Mus musculus 
2005-01-01 | E-MNIA-68 | biostudies-arrayexpress
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