Control (ROP9-3HA) parasites or PLP1-3HA expressing parasites were fixed with 0.1% PFA and lysed in HEPES-RIPA buffer (20 mM HEPES-NaOH, pH 7.5, 1 mM EGTA, 1 mM MgCI2, 150 mM NaCl, 0.25% sodium deoxycholate, 0.05% SDS, 1% NP-40) supplemented with protease inhibitor cocktail cOmplete EDTA-free (Roche...
ORGANISM(S): Cellular Organisms