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Essential Thrombocythemia (ET) patients, as well as normal donors BM CD34+ cells were differentiated in thrombopoietin-treated liquid suspension cultures. Briefly, CD34+ cells (80,000 /mL) were resuspended in a serum-free medium in the presence of 100 ng/mL of human recombinant thrombopoietin (TPO; ...
ORGANISM(S): Homo sapiens 
Normal donors BM CD34+ cells were differentiated in thrombopoietin-treated liquid suspension cultures. Briefly, CD34+ cells (80,000 /mL) were resuspended in a serum-free medium in the presence of 100 ng/mL of human recombinant thrombopoietin (TPO; Genzyme, Boston, MA). Every 3 days, viable cells wer...
ORGANISM(S): Homo sapiens 
Hematopoietic stem cell (HSC) subsets based on the CD34 expression and/or lineage markers expression
ORGANISM(S): Homo sapiens 
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