Sort   by:  
 Page size 
GenomicsGenome-wide mapping of human DNA-replication origins: levels of transcription at Orc1 sites regulate origin selection and replication timing
We report the genome-wide mapping of Orc1 binding-sites in mammals and their validation as active DNA-replication origins (ORIs). Orc1 sites are universally associated with transcription start sites (TSSs) of coding or non-coding RNAs. Transcription levels at the Orc1 sites directly correlate with r...
ORGANISM(S): Homo sapiens 
2012-09-18 | E-GEOD-37583 | biostudies-arrayexpress
GenomicsEpigenomic profiling of Non-small-cell Lung Cancer (NSCLC) cells with or without Eed
Polycomb repressive complexes (PRC) are frequently implicated in human cancer acting either as oncogenes or tumor suppressors. Here we show that PRC2 is a critical regulator of Kras-driven non-small-cell lung cancer (NSCLC) progression. Modulation of PRC2 by either Ezh2 overexpression or Eed deletio...
ORGANISM(S): Homo sapiens 
2016-01-15 | E-GEOD-61190 | biostudies-arrayexpress
ProteomicsTEAD inhibitor K-975 enhances KRASG12C inhibitor adagrasib-mediated cancer cell proliferation inhibition via dual cell cycle arrest
Here we investigate the effect of the dual inhibition, in KRASG12C mutated NSCLC cells, using the KRASG12Ci adagrasib (MRTX849) and the pan-TEAD inhibitor (TEADi) K-975. We show that K-975 enhances adagrasib-induced tumor cell growth inhibition in cancer cells. Mechanistically, we detect a downregul...
ORGANISM(S): Homo Sapiens (human) 
2023-05-12 | PXD039726 | JPOST Repository
TranscriptomicsG9a is essential for epigenetic silencing of K+ channel genes in acute-to-chronic pain transition
AIM: We performed RNA-sequencing experiments seeking genes whose expression changed due to nerve injury. In addition, we wanted to test whether inhibition of the methyl transferase G9a/GLP, that methylates H3K9me2, could reverse those expression changes due to nerve ligation. G9a/GLP methylase was p...
ORGANISM(S): Rattus norvegicus 
2015-11-02 | E-GEOD-59043 | biostudies-arrayexpress
GenomicsGenome-wide analysis of BRCA1 and PALB2 [ChIP-seq and RNA-seq]
Breast and ovarian cancer susceptibility genes, BRCA1 and PALB2 have enigmatic roles in cellular growth and mammalian development. While these genes are essential for growth during early developmental programs, inactivation later in adulthood leads to increased growth and formation of tumors, leadi...
ORGANISM(S): Homo sapiens 
2013-09-30 | E-GEOD-40591 | biostudies-arrayexpress
TranscriptomicsFunctional Identification of Critical Bmi1 target genes in Neural Progenitor and Malignant Glioma cells
In this study, we combined a genome-wide analysis of the Polycomb protein Bmi1 and an in vivo RNAi screening to identify critical targets whose repression in neural progenitor and Malignant Glioma cells enables normal and aberrant self-renewal. Bmi1 ChIP-sequencing generated in 2 primary adult mouse...
ORGANISM(S): Mus musculus 
2013-05-21 | E-GEOD-33912 | biostudies-arrayexpress
GenomicsDigital Restriction Enzyme Analysis of Methylation (DREAM) of SW48 colon cancer cell line genomic DNA.
We analyzed levels of 5-methyl cytosine nnnn CCCGGG target sites by sequential restriction digest by SmaI and XmaI enzymes, ligating Illumina adaptors to the restriction fragments and reading methylation-specific signatures at the ends of restriction fragments by paired ends Illumina high throughput...
ORGANISM(S): Homo sapiens 
2015-09-08 | E-GEOD-66296 | biostudies-arrayexpress
UnknownPediatric Moyamoya Disease and Syndrome in Italy: A Multicenter Cohort.
Not available
S-EPMC9120837 | biostudies-literature
UnknownEpilepsy Course and Developmental Trajectories in STXBP1-DEE.
Not available
S-EPMC9157582 | biostudies-literature
Sort   by:  
 Page size