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TranscriptomicsS phase and HU profiles in wild-type and mutant cells
Total S phase was measured for wild-type cells undergoing meiS and mitS. Early replication origins were mapped in mitS in wild-type cells, and in meiS for wild-type, sml1 delete, rec8 delete and spo11 delete cells. Comparative genome hybridization was used to measure the kinetics and final extent of...
ORGANISM(S): Saccharomyces cerevisiae 
2012-06-18 | E-GEOD-35662 | biostudies-arrayexpress
ProteomicsMechanisms of microbial magnetite oxidation by Sideroxydans lithotrophicus ES-1
Iron-oxidizing bacteria are widely found in natural and man-made environments where they influence varied biogeochemical cycles. Despite their prevalence, the mechanisms and Fe(II) substrates used by these organisms remain understudied. To date, there has been limited exploration of the ability of i...
ORGANISM(S): Sideroxydans Lithotrophicus (ncbitaxon:63745) 
2024-01-04 | MSV000093770 | MassIVE
ProteomicsQuantitative proteomics study of Sideroxydans lithotrophicus grown on solid and aqueous Fe (II)
The cells were grown on Fe(II)-citrate as the aqueous Fe(II) source and Fe(II)-smectite as the solid Fe(II) source. Cells were collected on a 0.2 um filter membrane (Millipore GPWP) by filtration. Then cells on the membrane were lysed by vortex in the lysis buffer containing 100 mM Tris/HCl, 10 mM E...
ORGANISM(S): Sideroxydans Lithotrophicus Es-1 (ncbitaxon:580332) 
2022-03-29 | MSV000089149 | MassIVE
TranscriptomicsSeparation of DNA replication from the assembly of break-competent meiotic chromosomes
The meiotic cell division reduces the chromosome number from diploid to haploid to form gametes for sexual reproduction. Although much progress has been made in understanding meiotic recombination and the two meiotic divisions, the processes leading up to recombination, including the prolonged pre-m...
ORGANISM(S): Saccharomyces cerevisiae 
2012-06-14 | E-GEOD-35667 | biostudies-arrayexpress
TranscriptomicsChromatin IP for Mcm2-7, Rec8, Hop1 and Red1
Mcm2-7 ChIP in pre-meiotic and pre-mitotic cells, axis factor ChIP in wild-type and replication compromised strains in meiosis Multiple studies of meiotic chromosomes were undertaken. To study DNA replication, the locations of replicative helicase (Mcm2-7) were mapped in pre-meiotic and pre-mitotic ...
ORGANISM(S): Saccharomyces cerevisiae 
2012-06-18 | E-GEOD-35658 | biostudies-arrayexpress
TranscriptomicsDSB formation in replication compromised cells
DSBs were mapped genome-wide by ssDNA enrichment in cdc6-mn replication comprimsied strains. We mapped DSB sites by detecting DSB-associated ssDNA enrichment on microarrays. To test the role of DNA replication in DSB formation, we mapped ssDNA in a cdc6-mn replication depleted strain. ssDNA was isol...
ORGANISM(S): Saccharomyces cerevisiae 
2012-06-18 | E-GEOD-35666 | biostudies-arrayexpress
TranscriptomicsDissecting physiological and transcriptional responses of nitric oxide to drought stress by increasing in vivo nitric oxide content in Arabidopsis
Nitric oxide (NO) is involved in all major environmental stresses. However, most of these understandings were mainly based on pharmacological study using NO modulator compounds. Recently, our studies together with others provided a new class of plant experimental system with specific in vivo NO rele...
ORGANISM(S): Arabidopsis thaliana 
2014-06-03 | E-GEOD-48474 | biostudies-arrayexpress
TranscriptomicsClinical Utility of Circulating MicroRNA Signatures for Breast Cancer Diagnosis (Gene expression)
Purpose: There is a quest for novel non-invasive diagnostic markers for the detection of breast cancer. The goal of this study is to identify circulating microRNA signatures using a cohort of Asian Chinese breast cancer patients, and to compare microRNA profiles between tumour and serum samples. E...
ORGANISM(S): Homo sapiens 
2013-09-06 | E-GEOD-42072 | biostudies-arrayexpress
TranscriptomicsClinical Utility of Circulating MicroRNA Signatures for Breast Cancer Diagnosis
This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
ORGANISM(S): Homo sapiens 
2013-09-06 | E-GEOD-42128 | biostudies-arrayexpress
TranscriptomicsNatural variation of transcriptional networks in Arabidopsis thaliana in response to salt stress
Arabidopsis ecotypes of Sha and Ler showed differences in tolerance to salinity stress. A previous study indicated that a premature stop codon resulting in a truncated Response to ABA and Salt 1 (RAS1) protein in Sha contributes to the increased salt tolerance relative to Ler ecotype. Sha exhibited ...
ORGANISM(S): Arabidopsis 
2014-01-01 | E-GEOD-40940 | biostudies-arrayexpress
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