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ProteomicsBiosynthesis and secretion of the microbial sulfated peptide RaxX and binding to the rice XA21 immune receptor
The rice immune receptor XA21 is activated by the sulfated microbial peptide RaxX (required for activation of XA21-mediated immunity X) produced by Xanthomonas oryzae pv. oryzae (Xoo). Mutational studies and targeted proteomics revealed that RaxX is processed and secreted by the protease/transporter...
ORGANISM(S): Xanthomonas Oryzae Pv. Oryzae 
2019-04-16 | PXD013389 | panorama
ProteomicsAn automated ‘cells-to-peptides’ sample preparation workflow for high-throughput, quantitative proteomic assays of microbes
Mass spectrometry–based quantitative proteomic analysis has proven valuable for clinical and biotechnology related research and development. Driving this value have been improvements in the sensitivity, resolution, and robustness of mass analyzers. However, manual sample preparation protocols are of...
ORGANISM(S): Escherichia Coli Rhodotorula Toruloides Pseudomonas Putida Kt2440 Saccharomyces Cerevisiae 
2019-08-23 | PXD014182 | panorama
ProteomicsA microbial supply chain for production of anti-cancer drug vinblastine
Monoterpene indole alkaloids (MIAs) are a diverse family of complex plant secondary metabolites with many medicinal properties, including the essential anti-cancer therapeutics vinblastine and vincristine. As MIAs are difficult to chemically synthesize, the world’s supply chain for vinblastine relie...
ORGANISM(S): Saccharomyces Cerevisiae 
2022-09-09 | PXD024976 | panorama
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