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MetabolomicsA Comprehensive and Ultrasensitive Isotope Calibration Method for Soil Amino Compounds Using Orbitrap Mass Spectrometry
Bound amino compounds (amino acid and amino sugar polymers) comprise a significant proportion (~40%) of soil organic nitrogen and therefore represent an essential source of nitrogen for plant and microbial nutrition. The analysis of their content and isotope enrichment still represents a significant...
2025-06-16 | MTBLS12550 | MetaboLights
MetabolomicsComparison of popular enrichment methods for untargeted in vitro metabolomics.

Introduction

Untargeted metabolomics is a popular method by which researchers measure a large portion of the metabolites present in a biological system at once. This approach usually results in complex data sets containing tens to hundreds of thousands of observations whi...

2025-08-11 | MTBLS12332 | MetaboLights
MetabolomicsIlluminating the dark space of neutral glycosphingolipidome by selective enrichment and profiling at multi-structural levels

Glycosphingolipids (GSLs) are essential components of cell membranes, particularly enriched in the nervous system. Altered molecular distributions of GSLs are increasingly associated with human diseases, emphasizing the significance of lipidomic profiling. Traditional GSL analysis methods are ham...

2024-06-25 | MTBLS10400 | MetaboLights
MetabolomicsEvaluation of extraction solvents for untargeted metabolomics analysis of enrichment reactor cultures performing enhanced biological phosphorus removal (EBPR)

INTRODUCTION: The extraction solvent mixtures were optimized for untargeted metabolomics analysis of microbial communities from two laboratory scale activated sludge reactors performing enhanced biological phosphorus removal (EBPR).

OBJECTIVE: To develop a ...

2019-08-16 | MTBLS884 | MetaboLights
MetabolomicsPositional Enrichment by Proton Analysis (PEPA): a One-Dimensional 1H-NMR Approach for 13C Stable Isotope Tracer Studies in Metabolomics
A novel metabolomics approach for NMR-based stable isotope tracer studies called PEPA is presented, and its performance validated using human cancer cells. PEPA detects the position of carbon label in isotopically enriched metabolites and quantifies fractional enrichment by indirect determination of...
2017-02-23 | MTBLS247 | MetaboLights
ProteomicsArginine dimethylation and liquid-liquid phase separation
Recently, arginine methylation was found to regulate the protein LLPS which drives the formation of MLOs. Here, we developed a chemical enrichment method coupled with LC-MS/MS to analyze dynamic change of arginine di-methylation upon SG formation.
ORGANISM(S): Homo sapiens (Human) 
2022-10-20 | PXD033048 | Pride
Liquid-liquid phase separation Arginine dimethylation Chemical enrichment method Stress granule Rg/rgg motifs 
ProteomicsArginine dimethylation and liquid-liquid phase separation_Supplementary
Recently, arginine methylation was found to regulate the protein LLPS which drives the formation of MLOs. Here, we developed a chemical enrichment method coupled with LC-MS/MS to analyze dynamic change of arginine di-methylation upon SG formation.
ORGANISM(S): Homo sapiens (Human) 
2022-10-20 | PXD035137 | Pride
Liquid-liquid phase separation Arginine dimethylation Chemical enrichment method Stress granule Rg/rgg motifs 
GenomicsSelective chemical labeling reveals intragenic enrichment of 5-hydroxymethylcytosine in mouse cerebellum
5-Methylcytosine (5-mC) is an important DNA modification found in eukaryotes that impacts gene regulation and disease pathogenesis. Recently, 5-hydroxymethylcytosine (5-hmC), another form of DNA modification, has been identified in substantial amounts in certain mammalian cell types; however, its ro...
ORGANISM(S): Mus musculus 
2010-12-12 | E-GEOD-25398 | biostudies-arrayexpress
ProteomicsAn automated intact glycopeptide enrichment method for serum site-specific N-glycoproteomic analysis
We developed an automated glycopeptide enrichment method for the analysis of serum site-specific N-glycoproteome. This automated method allowed for processing one sample within 20 min. It showed higher enrichment specificity, more intact glycopeptide identifications, and better quantitative reproduc...
ORGANISM(S): Homo Sapiens (human) 
2021-04-06 | PXD025151 | JPOST Repository
ProteomicsTransforming chemical proteomics into high-throughput method using SP2E workflow.
Protein post-translational modifications (PTMs) play a critical role in regulation of protein catalytic activity, localization and protein-protein interactions. An attachment of PTMs onto proteins significantly diversify their structure and function resulting in so called proteoforms. However, the s...
ORGANISM(S): Homo sapiens (Human) 
2022-07-04 | PXD033644 | Pride
Method development Ampylation 
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