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Metabolomics13C glucose labelling studies using 2D NMR is a useful tool for determining ex vivo whole organ metabolism during hypothermic machine perfusion of kidneys
Background: The aim of this study is to determine the feasibility of using Nuclear Magnetic Resonance (NMR) tracer studies (13C-enriched glucose) to detect ex vivo de novo metabolism in the perfusion fluid and cortical tissue of porcine kidneys during hypothermic machine perfusion (HMP). Methods: Po...
2017-10-16 | MTBLS367 | MetaboLights
MetabolomicsMatrix Effect on Chemical Isotope Labeling and Its Implication in Metabolomic Sample Preparation for Quantitative Metabolomics
Matrix effect from various constituents in biological samples can reduce the accuracy of quantitative metabolomics. Differential chemical isotope labeling liquid chromatography mass spectrometry (CIL LC-MS) can overcome the matrix effect on MS detection based on measuring the intensity ratios of met...
2015-07-01 | MTBLS194 | MetaboLights
TranscriptomicsDirect reprogramming of mouse fibroblasts into cardiomyocytes with chemical cocktails
The direct conversion, or trans-differentiation, of non-cardiac cells into cardiomyocytes by forced expression of transcription factors and microRNAs provide promising ways of cardiac regeneration. However, genetic manipulations are still not desirable in real clinical applications. we report the ge...
ORGANISM(S): Mus musculus 
2015-08-23 | E-GEOD-69924 | biostudies-arrayexpress
ProteomicsEvaluation of NHS-Acetate and DEPC labelling for determination of solvent accessible amino acid residues in protein complexes
The structural analysis of proteins and protein complexes is key for understanding their function and regulation. In order to gain structural information, we examined protein surface labelling using NHS-Acetate and DEPC following an MS-based workflow. Using two model proteins we characterise the obt...
ORGANISM(S): Oryctolagus cuniculus cuniculus Saccharomyces cerevisiae (Baker's yeast) 
2020-05-05 | PXD015940 | Pride
Diethylpyrocarbonate N-hydroxysuccinimidyl acetate Mass spectrometry Neutral loss Chemical labelling Solvent accessible surface area Labelling percentage 
TranscriptomicsAssessment of chemical mixtures and ground water effects on Daphnia magna transcriptomics
Small organisms can be used as biomonitoring tools to assess chemicals in the environment. Chemical stressors are especially hard to assess and monitor when present as complex mixtures. Here, Daphnia magna were exposed for 24 hours to five different munitions constituents 2,4,6-trinitrotoluene (TNT)...
ORGANISM(S): Daphnia magna 
2011-06-22 | E-GEOD-30163 | biostudies-arrayexpress
ProteomicsDNA-guided Photoactivatable Probe-based Chemical Proteomics Reveals the Reader Protein of mRNA Methylation
We report a DNA templated chemistry and photo-crosslinking based method to detect the readers of mRNA modification. By use of N6-methyladenosine (m6A), we illustrated that this method can be successfully utilized for labelling and enriching the readers of mRNA modification, as well as for the discov...
ORGANISM(S): Homo Sapiens 
2020-05-30 | PXD019480 |
UnknownChemical labelling for visualizing native AMPA receptors in live neurons.
Not available
S-EPMC5385570 | biostudies-literature
TranscriptomicsGeneration of neural progenitor cells by chemical cocktails and hypoxia
Neural progenitor cells (NPCs) can be induced from somatic cells by defined factors. Here we report that NPCs can be generated from mouse embryonic fibroblasts by a chemical cocktail, namely VCR (V, VPA, an inhibitor of HDACs; C, CHIR99021, an inhibitor of GSK-3 kinases and R, Repsox, an inhibitor o...
ORGANISM(S): Mus musculus 
2015-04-27 | E-GEOD-68311 | biostudies-arrayexpress
UnknownClick-based amplification: designed to facilitate various target labelling modes with ultralow background amplification.
Not available
S-EPMC8341700 | biostudies-literature
GenomicsDirect detection of circulating microRNA-122 using dynamic chemical labelling with single molecule detection overcomes stability and isomiR challenges for biomarker qualification
Direct detection of circulating microRNA-122 using dynamic chemical labelling with single molecule detection overcomes stability and isomiR challenges for biomarker qualification
PRJNA591119 | ENA
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