Sort   by:  
 Page size 
TranscriptomicsProbing the initiation and effector phases of the somatic piRNA pathway in Drosophila
Combining RNAi in cultured cells and analysis of mutant animals, we probed roles of known piRNA pathway components in the initiation and effector phases of transposon silencing. total RNA and RNA associated with Piwi was isolated and size-fractionated by PAGE into 19-29nt. These were processed and s...
ORGANISM(S): Drosophila melanogaster 
2010-10-25 | E-GEOD-24108 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human ESC line derived neural progenitor cells (hNPCs)
The self-renewal and differentiation potential of human embryonic stem cells (hESCs) suggests that hESCs could be used for regenerative medicine, especially for restoring neuronal functions in brain diseases. However, the functional properties of neurons derived from hESC are largely unknown. Moreov...
ORGANISM(S): Homo sapiens 
2008-06-16 | E-GEOD-8347 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mice hearts comparing microRNA-22 overexpression to wildtype
To explore the role of miR-22 in the heart, we generated miR-22 null and transgenic mice. Cardiac transgenic overexpression of miR-22 in vivo led to cardiac hypertrophy that evolved into progressive dilated cardiomyopathy (DCM) in unstressed hearts. We found that miR-22 directly regulates two transc...
ORGANISM(S): Mus musculus 
2012-12-01 | E-MTAB-501 | biostudies-arrayexpress
TranscriptomicsA genome-wide RNAi screen draws a genetic framework for transposon control and primary piRNA biogenesis in Drosophila
A large fraction of our genome consists of mobile genetic elements. Governing transposons in germ cells is critically important, and failure to do so compromises genome integrity, leading to sterility. In animals, the piRNA pathway is the key to transposon constraint, yet the precise molecular detai...
ORGANISM(S): Drosophila melanogaster 
2013-05-09 | E-GEOD-46009 | biostudies-arrayexpress
TranscriptomicsAnalysis of Gsk-3-Deficient Embryonic Stem Cells Reveals Large-Scale Alterations in Gene Expression
The two vertebrate Gsk-3 isoforms, Gsk-3a and Gsk-3b, are encoded by distinct genetic loci and exhibit mostly redundant function in murine embryonic stem cells (ESCs). Here we report that deletion of both Gsk-3a and Gsk-3b in mouse ESCs results in significant changes in gene expression. In contrast,...
ORGANISM(S): Mus musculus 
2011-09-14 | E-GEOD-27395 | biostudies-arrayexpress
Sort   by:  
 Page size