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We first overexpressed RNF157 in Huh-7 cells, followed by collection of the cells and lysis in a lysis buffer (RIPA) supplemented with a protease inhibitor cocktail. Then the cell lysate was centrifuged at 4℃ for 10 minutes to remove cell precipitation. Boil and perform western blot analysis.
ORGANISM(S): Homo sapiens (Human) 
2024-10-21 | PXD052805 | Pride
Label-free proteome analysis of small extracellular vesicles (sEV) derived from WM9 cells expressing different HRS mutants: wild type (HRSWT), phospho-deficient (HRSS345A), and phospho-mimetic mutant HRS (HRSS345D).
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2022-02-16 | MSV000088846 | MassIVE
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