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MetabolomicsMetabolomic Responses of Arabidopsis Suspension Cells to Bicarbonate under Light and Dark Conditions
Global CO2 level presently recorded at 400 ppm is expected to reach 550 ppm in 2050, an increment likely to impact plant growth and productivity. Using targeted LC-MS and GC-MS platforms we quantified 229 and 29 metabolites, respectively in a time-course study to reveal short-term responses to diffe...
2017-04-25 | MTBLS311 | MetaboLights
MetabolomicsJasmonate-mediated stomatal closure under elevated CO2 revealed by time-resolved metabolomics
Foliar stomatal movements are critical for regulating plant water loss and gas exchange. Elevated carbon dioxide (CO2) levels are known to induce stomatal closure. However, the current knowledge on CO2 signal transduction in stomatal guard cells is limited. Here we report metabolomic responses of Br...
2017-04-25 | MTBLS312 | MetaboLights
ProteomicsMPK phosphorylation of CDKC - Modulation of RNA polymerase II phosphorylation downstream of pathogen perception orchestrates plant immunity
phosphorylation site mapping of CDKC proteins, which were phosphorylated by MPK3 or MPK6
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-06-04 | PXD001177 | Pride
Mpk Cdkc 
MetabolomicsMetabolomics of red-light-induced stomatal opening in Arabidopsis thaliana: Coupling with abscisic acid and jasmonic acid metabolism
Environmental stimuli-triggered stomatal movement is a key physiological process that regulates CO2 uptake and water loss in plants. Stomata are defined by pairs of guard cells that perceive and transduce external signals, leading to cellular volume changes and consequent stomatal apertur...
2022-01-21 | MTBLS1326 | MetaboLights
MetabolomicsMetabolomic Responses of Guard Cells and Mesophyll Cells to Bicarbonate
Anthropogenic CO2 presently at 400 ppm is expected to reach 550 ppm in 2050, an increment expected to affect plant growth and productivity. Paired stomatal guard cells (GCs) are the gate-way for water, CO2, and pathogen, while mesophyll cells (MCs) represent the bulk cell-type of green leaves mainly...
2017-03-31 | MTBLS309 | MetaboLights
ProteomicsCreation of a Plant Metabolite Spectral Library for Untargeted and Targeted Metabolomics
Large-scale high throughput metabolomic technologies are indispensable components of systems biology in terms of discovering and defining the metabolite parts of the system. However, the lack of a plant metabolite spectral library limited the metabolite identification of plant metabolomics study. He...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2022-12-03 | MSV000090829 | MassIVE
ProteomicsMPK4 interacting proteins in guard cells through AP-MS
Plants are often challenged by numerous biotic and abiotic environmental stresses. They have evolved efficient signaling networks in response to the environmental challenges, such as pathogen invasion, drought, CO2 changes. Guard cells are specialized epidermal cells enclosing pores on leaf surface,...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-04-30 | PXD034347 | Pride
Ap-ms Arabidopsis thaliana Mpk4 Guard cells Single-cell-type proteomics 
ProteomicsControl and LgG4 binding proteins
Proteins were captured with monosaccharide(Control) and LgG4 probe. Resulting proteins were went through proteomics. Unique proteins found in LgG4 samples were selected for further research.
ORGANISM(S): Homo sapiens (Human) 
2024-04-30 | PXD030528 | Pride
Lgg4 probe Hela cell 
ProteomicsMPK4 phosphorylation dynamics and interacting proteins in plant immunity
The Arabidopsis MAP kinase 4 (MPK4) has been proposed as negative player in plant immunity, but it is activated by pathogen-associated molecular patterns (PAMPs), such as flg22. The molecular mechanisms by which MPK4 is activated and controls plant defense remain elusive. In this study, we investiga...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-01-14 | PXD009016 | Pride
Arabidopsis mpk4  innate immunity  protein-protein interaction  flg22  kinase substrate  ip-ms 
ProteomicsMitogen-activated protein kinase 4 regulated metabolic networks
Mitogen-activated protein kinase 4 (MPK4) was first identified as a negative regulator of systemic acquired resistance (SAR). It is also an important kinase that gets involved in other plant biological processes in plants, including cytokinesis, reproduction and photosynthesis. Arabidopsis thaliana ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-02-15 | PXD030175 | Pride
Arabidopsis thaliana Mpk4 
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