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ProteomicsHEK293T 15xGly/99xGly immunoprecipitation-mass spectrometry
In order to determine the protein composition of polyglycine aggregates, clonal HEK293T cell lines harboring doxycycline (DOX)-suppressible 15xGly-eGFP-3xHA or 99xGly-eGFP-3xHA constructs were grown either in standard media for 14 days (induction of transgene) or maintained in DOX (suppression of tr...
ORGANISM(S): Homo sapiens (Human) 
2025-07-18 | PXD046400 | Pride
Ggc repeat Polyglycine 
ProteomicsHEK293T 15xGly/99xGly soluble quantitative proteomics
In order to determine the protein composition of polyglycine aggregates, clonal HEK293T cell lines harboring doxycycline (DOX)-suppressible 15xGly-eGFP-3xHA or 99xGly-eGFP-3xHA constructs were grown either in standard media for 14 days (induction of transgene) or maintained in DOX (suppression of tr...
ORGANISM(S): Homo sapiens (Human) 
2025-07-18 | PXD046427 | Pride
Ggc repeat Polyglycine 
TranscriptomicsAnalysis of miR-143/145 wild-type and null mouse intestinal myofibroblasts before and after LPS stimulation
In a series of mouse genetic studies, we concluded that miR-143/145 expression in intestinal subepithelial myofibroblasts (ISEMFs) promotes epithelial regeneration after DSS-mediated injury in the colon. This experiment aims to identify miR-143/145 target genes that are involved in this function. We...
ORGANISM(S): Mus musculus 
2014-05-22 | E-GEOD-55651 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human 493-6 cells (human EBV-transformed B lymphocytes) were infected with a retrovirus that produces human Lin-28B or empty virus to identify Myc- and Lin-28B-responsive transcripts
In order to examine the consequences of Myc and Lin-28B induction on the transcriptome, P493-6 cells (human EBV-transformed B lymphocytes) were infected with a retrovirus that produces human Lin-28B or empty virus as a control. Gene expression profiles were then monitored in the high and low Myc sta...
ORGANISM(S): Homo sapiens 
2009-03-14 | E-GEOD-14302 | biostudies-arrayexpress
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