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TranscriptomicsIdentifying crossover locations in an Arabidopsis thaliana wild type Col x Ler F2 and meiMIGS-PPX1-PPX2 Col x Ler F2 population using genotyping by sequencing
Genomic DNA from 144 wild type Col x Ler F2 individual and 96 meiMIGS-PPX1-PPX2 Col x Ler F2 individuals was extracted using CTAB and used to generate sequencing libraries as described (Rowan et al; Yelina et al), with the following modifications. DNA was extracted from 3 rosette leaves of 5 week ol...
ORGANISM(S): Arabidopsis thaliana 
2021-03-12 | E-MTAB-10168 | biostudies-arrayexpress
TranscriptomicsRegulation of microRNA-mediated developmental changes by the SWR1 chromatin remodeling complex in Arabidopsis thaliana
We investigated small-RNA populations from Arabidopsis thaliana Col-0 between Wild-type and the arp6 mutant with focus on micro-RNA levels. Small-RNA sequencing was performed for 4 bioreps (with 4 technical replicates each) of each condition. Size selection was done by manually cutting bands.
ORGANISM(S): Arabidopsis thaliana 
2016-05-31 | E-MTAB-4498 | biostudies-arrayexpress
GenomicsIdentifying crossover locations from pollen of Arabidopsis thaliana Col x Ler F1 plants (WT) using Nanopore-seq and COmapper
We sequenced pollen DNA from Col x Ler F1 hybrid plants using Nanopore long-read sequencing and identified crossover sites with COmapper. To purify pollen grains from F1 hybrid plants and extract their genomic DNA, whole inflorescences of hybrid plants were collected and transferred to ice-cold 10% ...
ORGANISM(S): Arabidopsis thaliana 
2025-05-17 | E-MTAB-15083 | biostudies-arrayexpress
GenomicsIdentifying crossover locations from pollen of Arabidopsis thaliana Col x Ler F1 plants (WT and recq4) using Nanopore-seq and COmapper
We sequenced pollen DNA from Col x Ler F1 hybrid plants using Nanopore long-read sequencing and identified crossover sites with COmapper. To purify pollen grains from F1 hybrid plants and extract their genomic DNA, whole inflorescences of hybrid plants were collected and transferred to ice-cold 10% ...
ORGANISM(S): Arabidopsis thaliana 
2024-08-19 | E-MTAB-14369 | biostudies-arrayexpress
UnknownHIGH CROSSOVER RATE1 encodes PROTEIN PHOSPHATASE X1 and restricts meiotic crossovers in Arabidopsis.
Not available
S-EPMC7610654 | biostudies-literature
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