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TranscriptomicsNative elongating transcript sequencing (NET-seq) of wild type Saccharomyces cerevisiae and of DST1, RCO1, SET1, SET2, EAF3 deletion strains
We present an approach (native elongating transcript sequencing, NET-seq), based on deep sequencing of 3M-bM-^@M-^Y ends of nascent transcripts associated with RNA polymerase, to monitor transcription at nucleotide resolution. Application of NET-seq in Saccharomyces cerevisiae reveals that while pro...
ORGANISM(S): Saccharomyces cerevisiae 
2011-01-19 | E-GEOD-25107 | biostudies-arrayexpress
TranscriptomicsNET-seq of S. cerevisiae cells after knocking-out genes encoding various mRNA decay factors
NET-seq was performed on yeast strains carrying deletions of mRNA decay factors (plus a control) in order to assess the effect of their deletion on RNA polymerase II occupancy at genetic loci. This is in pursuit of understanding the process of mRNA buffering which links mRNA synthesis and decay.
ORGANISM(S): Saccharomyces cerevisiae 
2020-02-08 | E-MTAB-8749 | biostudies-arrayexpress
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