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TranscriptomicsNative Elongating Transcript sequencing (NET-seq) in wild-type and three members of the CAF-I complex.
We performed a fluorescent reporter based screen to identify factors determining transcriptional directionality from bidirectional promoters that give rise to a coding and a non-coding transcript. Promoters like these are most frequent in many organisms and non-coding transcription from this origin ...
ORGANISM(S): Saccharomyces cerevisiae 
2014-06-01 | E-GEOD-55982 | biostudies-arrayexpress
TranscriptomicsNET-seq of S. cerevisiae cells after knocking-out genes encoding various mRNA decay factors
NET-seq was performed on yeast strains carrying deletions of mRNA decay factors (plus a control) in order to assess the effect of their deletion on RNA polymerase II occupancy at genetic loci. This is in pursuit of understanding the process of mRNA buffering which links mRNA synthesis and decay.
ORGANISM(S): Saccharomyces cerevisiae 
2020-02-08 | E-MTAB-8749 | biostudies-arrayexpress
TranscriptomicsNative elongating transcript sequencing (NET-seq) of wild type Saccharomyces cerevisiae and of DST1, RCO1, SET1, SET2, EAF3 deletion strains
We present an approach (native elongating transcript sequencing, NET-seq), based on deep sequencing of 3M-bM-^@M-^Y ends of nascent transcripts associated with RNA polymerase, to monitor transcription at nucleotide resolution. Application of NET-seq in Saccharomyces cerevisiae reveals that while pro...
ORGANISM(S): Saccharomyces cerevisiae 
2011-01-19 | E-GEOD-25107 | biostudies-arrayexpress
GenomicsTranslational activators align mitochondrial mRNAs at the small ribosomal subunit for translation initiation
Translational activators align mitochondrial mRNAs at the small ribosomal subunit for translation initiation
PRJNA1191052 | ENA
GenomicsA molecular switch at the yeast mitoribosomal tunnel exit controls cytochrome b synthesis
A molecular switch at the yeast mitoribosomal tunnel exit controls cytochrome b synthesis
PRJNA1192275 | ENA
GenomicsSaccharomyces cerevisiae
Synchronized translation programs across cellular compartments
PRJNA300880 | ENA
GenomicsProteome-scale movements and compartment connectivity during the eukaryotic cell cycle
Proteome-scale movements and compartment connectivity during the eukaryotic cell cycle
PRJNA1003962 | ENA
GenomicsDynamics of transcription elongation are finely-tuned by dozens of regulatory factors
Dynamics of transcription elongation are finely-tuned by dozens of regulatory factors
PRJNA669852 | ENA
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