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ProteomicsCXCL12 restricts tumor growth by suppressing the Ras, ERK1/2, c-Myc and the immune checkpoint PDL1 pathways
Cytokines constitute a family of proteins that are secreted by a broad variety of cells and modulate the immune response. CXCL12, along with its receptor CXCR4, are essential players in numerous biological processes. Dysregulation of their function can underlie the mechanisms(s) of several pathologi...
ORGANISM(S): Homo sapiens (Human) 
2024-11-28 | PXD054783 | Pride
Pdl1 Tumor suppression Cxcl12 
ProteomicsDIA-PASEF–based total proteome profiling of HeLa cells expressing nuclear or cytosolic p62 condensates
Cells were lysed in 6 M guanidine-HCl, 100 mM HEPES-NaOH, pH7.5, 10 mM TCEP, and 40 mM CAA. The lysates were dissolved by heating and sonication, followed by centrifugation at 20,000 × g for 15 min at 4 °C. The supernatants were recovered, and proteins (20 µg each) were purified by the SP3 method an...
ORGANISM(S): Homo Sapiens (human) 
2025-12-30 | PXD072518 | JPOST Repository
ProteomicsDIA-PASEF–based FLAG-IP proteomic analysis of nuclear or cytosolic p62 condensates in HeLa cells
Cells were cross-linked with 0.1% formaldehyde for 10 min at 25 °C and quenched with 100 mM glycine. After washing with HBS (20 mM HEPES-NaOH, pH 7.5, 150 mM NaCl), cells were lysed in HEPES-RIPA buffer (20 mM HEPES-NaOH, pH 7.5, 150 mM NaCl, 1 mM MgCl₂, 1 mM EGTA, 1% NP-40, 0.25% sodium deoxycholat...
ORGANISM(S): Homo Sapiens (human) 
2025-12-30 | PXD072519 | JPOST Repository
ModelsNguyenLK2011 - Ubiquitination dynamics in Ring1B/Bmi1 system
NguyenLK2011 - Ubiquitination dynamics in Ring1B-Bmi1 system This theoretical model investigates the dynamics of Ring1B/Bmi1 ubiquitination to identify bistable switch-like and oscillatory behaviour in the system. Michaelis-Menten (MM) equations are used to formulate the model. However, the authors ...
2024-09-02 | BIOMD0000000622 | BioModels
ProteomicsFBXO7 Binds to and Increases the Expression of the p105 Subunit of NF-kB
Cell lysate from HAP1 cells were separated on several columns, the proteins were digested with trypsin and analysed on a Q Exactive HF-X instrument.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-08-05 | MSV000085912 | MassIVE
TranscriptomicsProfile of gene expression in U87-MG xenografts expressing control vector (V0), the ubiquitin ligase KPC1 or the p50 subunit of the NF-kB transcription factor, using RNASeq analysis of transcripts mapped independently to the human and murine genomes
Purpose: We identified KPC1 as the ubiquitin ligase that binds to the p105 precursor of NF-kB, ubiquitinates it and mediates its proteasomal processing to generate the p50 active subunit of the transcription factor. Using U87-MG human glioblastoma xenografts, we observed that overexpression of KPC1 ...
ORGANISM(S): Homo sapiens 
2015-04-09 | E-GEOD-60530 | biostudies-arrayexpress
ProteomicsProteomics analysis of HeLa cells starved with or without MG132
HeLa cells were amino acid starved for 8 hours in the presence or absence of MG132 or untreated. Cells were lysed by addition of HEPES 50 mM pH7.9 with 5% SDS. Proteins were precipitated with methanol/chloroforme and the proteins were resolubilized in 8 M Urea and digested with trypsin. The peptides...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-03-30 | MSV000083644 | MassIVE
ProteomicsRegulation of proteome by the proteasome upon ER stress
HeLa cells were with Tunicamycin or Thapsigargin for 8 hours in the presence or absence of MG132 or untreated. Cells were lysed by addition of HEPES 50 mM pH7.9 with 5% SDS. Proteins were precipitated with methanol/chloroforme and the proteins were resolubilized in 8 M Urea and digested with trypsin...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-04-30 | MSV000083736 | MassIVE
ProteomicsProteomics of HeLa cells starved and treated with YWF or leptomycin B
HeLa cells labeled with SILAC and were amino acids starved and treated with YWF, leptomycin B, MG132 or chloroquine. Cells were lysed with a 8M Urea buffer and proteins were reduced and alkylated. Equal amount of proteins of different labels were mixed and were digested with trypsin and resulting pe...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-08-01 | MSV000092564 | MassIVE
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