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ProteomicsMonitor precursor mass shifts of Halobacterium salinarum
Approximately 2x106 CL1-5 and H23 cells were seeded on 10-cm dishes with RPMI-1640 medium containing 10% fetal bovine serum. After the cells covered 80% of the dish, cells were serum-starved for 24 h in RPMI-1640 medium. CL1-5 and H23 cell lysates were harvested, trypsinized and analyzed by using LT...
ORGANISM(S): Homo sapiens (Human) Halobacterium salinarum 
2020-01-14 | PXD000082 | Pride
Cl1-5 lysate Nrc-1 gvd lysate Nrc-1 lysate Ltq-orbitrap xl Ltq-orbitrap discovery H23 lysate Human nsclc cell line Halobacterium salinarum nrc-1 Lc-ms/ms 
ProteomicsNAIA for cysteine profiling in HepG2 cells, protein target identification of covalent ligand and studying cysteine oxidative modifications
New cysteine probes, N-acryloylindole-alkynes (NAIAs), have been developed. We have applied NAIA-5 for cysteine profiling in HepG2 cell lysates, and the results have been compared to the conventional cysteine probe iodoacetamide-alkyne (IAA). NAIA-5 allows identification of more ligandable Cys than ...
ORGANISM(S): Homo sapiens (Human) 
2023-06-14 | PXD041264 | Pride
Profiling ligandable cysteines and studying cysteine oxidative modifications in human liver cancer cells hepg2 by naia-5 using bruker timstof ms 
TranscriptomicsH3K9K14ac ChIP-chip in lung cancer cells treated with histone deacetylase inhibitor
Lung cancer is the leading cause of cancer mortality worldwide, yet the therapeutic strategy for advanced non-small cell lung cancer (NSCLC) is limitedly effective. In addition, validated histone deacetylase (HDAC) inhibitors for the treatment of solid tumors remain to be developed. Here, we propose...
ORGANISM(S): Homo sapiens 
2010-12-22 | E-GEOD-20304 | biostudies-arrayexpress
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