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ProteomicsClick-linking: a cell-compatible protein crosslinking method based on click chemistry
We developed a novel approach to in situ crosslinking mass spectrometry (XL-MS) where the reaction is split into two sequential and orthogonal coupling events. The method involves pre-stabilization of the proteome using a fixation protocol, followed by a two-step process that begins with extensive l...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2025-01-28 | PXD059118 | Pride
In situ Crosslinking Proteome-wide Protein-protein interactions Interactome 
GenomicsIdentification of newly synthesized proteins in microbial communities using BONCAT and click chemistry
Identification of newly synthesized proteins in microbial communities using BONCAT and click chemistry
PRJEB70937 | ENA
ProteomicsTracing active members in microbial communities by BONCAT and click chemistry-based enrichment of newly synthesised proteins
Metaproteomics enables the description of microbial communities (MC). Microbial adaptation to changing environments is conducted by expressing newly synthesized proteins (nP) that can be difficult to distinguish from background proteins. Focusing on nP would add a new dimension to the metaproteomics...
ORGANISM(S): Escherichia coli (strain K12 / W3110 / ATCC 27325 / DSM 5911) Saccharomyces cerevisiae (Baker's yeast) anaerobic digester metagenome 
2024-10-17 | PXD047252 | Pride
Boncat Anaerobic digestion Metaproteomics Microbiome Mass spectrometry Click chemistry 
ProteomicsEnhanced protein-protein interaction network construction promoted by in vivo cross-linking with acid-cleavable click chemistry enrichment
Chemical cross-linking coupled with mass spectrometry has emerged as a powerful strategy which enables global profiling of protein interactome with direct interaction interfaces in complex biological systems. The alkyne-tagged enrichable cross-linkers are preferred to improve the coverage of low-abu...
ORGANISM(S): Homo Sapiens (human) 
2022-07-14 | PXD035317 | JPOST Repository
GenomicsClick chemistry enables comprehensive preclinical evaluation of targeted epigenetic therapies [Click-Seq 2]
The success of targeted therapies hinges on our ability to understand the molecular and cellular mechanism of action of these agents. Here we modify various BET bromodomain inhibitors, an exemplar novel targeted therapy, to create functionally conserved compounds that are amenable to click-chemistry...
ORGANISM(S): Homo sapiens 
2017-07-13 | GSE88749 | GEO
GenomicsClick chemistry enables comprehensive preclinical evaluation of targeted epigenetic therapies [Click-Seq 1]
The success of targeted therapies hinges on our ability to understand the molecular and cellular mechanism of action of these agents. Here we modify various BET bromodomain inhibitors, an exemplar novel targeted therapy, to create functionally conserved compounds that are amenable to click-chemistry...
ORGANISM(S): Homo sapiens 
2017-07-13 | GSE88748 | GEO
ProteomicsIdentification of Cytosolic Protein Targets of Catechol Estrogens in Breast Cancer Cells Using a Click Chemistry-based Workflow
Catechol estrogens (CEs) are known to be toxic metabolites and the initiators in the oncogenesis of breast cancers via forming covalent adducts with DNAs. CEs shall also react with proteins but their cellular protein targets remain un-explored. Here we reported the identification of protein targets ...
ORGANISM(S): Homo sapiens (Human) 
2020-09-28 | PXD017731 | Pride
Affinity purification Catechol estrogens Breast cancer cells Mass spectrometry Proteomic 
GenomicsSingle-cell nascent RNA sequencing using click-chemistry unveils coordinated transcription
Single-cell nascent RNA sequencing using click-chemistry unveils coordinated transcription
PRJNA1011911 | ENA
ProteomicsIdentification of substrates of ZDHHC19 in mouse haploid spermatids using click chemistry
Reversible palmitoylation is catalyzed by palmitoyltransferase(PAT) from the zinc-finger DHHC domain-containing protein (ZDHHC) family. The Zdhhc19 knockout male mice showed infertility and Zdhhc19 deficient sperm showed severe morphological defects. To identify the candidate substrates of ZDHHC19 i...
ORGANISM(S): Mus musculus (Mouse) 
2025-09-10 | PXD057727 | Pride
Spermiognesis  zdhhc19  palmitoylation 
ProteomicsIdentification of O-GlcNAcylated proteins and mapping of O-GlcNAc sites using Click Chemistry following extensive fractionation of extracted proteins
The O-N-acetyl-β-D-glucosaminylation, termed O-GlcNAcylation, is an atypical glycosylation corresponding to the transfer of a unique saccharide, the N-acetyl-β-D-glucosamine, on the hydroxyl group of serine and threonine amino acids of nuclear, cytosolic and mitochondrial proteins. Because of its dy...
ORGANISM(S): Mus musculus (Mouse) 
2018-08-21 | PXD004860 | Pride
Muscle Mouse O-glcnacylation Lc_msms Sites mapping Post-translational modifications C2c12 muscle cells 
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