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MetabolomicsLiquid chromatography coupled to high-resolution mass spectrometry metabolomics: A useful tool for investigating tumor secretome based on a three-dimensional co-culture model

Three-dimensional (3D) cell culture technologies, which more closely mimic the complex microenvironment of tissue, are being increasingly evaluated as a tool for the preclinical screening of clinically promising new molecules, and studying of tissue metabolism. Studies of metabolites released int...

2023-12-12 | MTBLS5375 | MetaboLights
MetabolomicsMetabolomics revealed the influence of breast cancer on lymphatic endothelial cell metabolism, metabolic crosstalk, and lymphangiogenic signaling in co-culture

Breast cancer metastasis occurs via blood and lymphatic vessels. Breast cancer cells 'educate' lymphatic endothelial cells (LECs) to support tumor vascularization and growth. However, despite known metabolic alterations in breast cancer, it remains unclear how lymphatic endothelial cell metabolis...

2024-06-25 | MTBLS859 | MetaboLights
MetabolomicsInvestigating the Impact of Acinetobacter lwoffii-ECT1/E6E7 Co-culture Metabolites on Cervical Cell Proliferation and Apoptosis via Untargeted Metabolomics.

This study employed untargeted metabolomics detection combined with bioinformatics analysis to investigate the metabolites from the co-culture of Acinetobacter lwoffiiand cervical precancerous cells ECT1/E6E7. A. lwoffiiwas co-cultured with ECT1/E6E7 cells at an MOI of 100. The culture supernatan...

2025-12-17 | MTBLS13526 | MetaboLights
MetabolomicsA microfluidics-based in vitro model of the gastrointestinal human-microbe interface
Changes in the human gastrointestinal microbiome are associated with several diseases. To infer causality, experiments in representative models are essential, but widely used animal models exhibit limitations. Here we present a modular, microfluidics-based model (HuMiX, human–microbial crosstalk), w...
2017-07-28 | MTBLS328 | MetaboLights
MetabolomicsMass Spectrometry Imaging of Lipids in a Gut Epithelial Cell Model

Scope: The Caco2/HT29-MTX co-culture system is widely used as a cell model of the intestinal epithelium. Although the gut epithelium plays an important role in the uptake of free fatty acids and the resynthesis of triglycerides, the lipid distribution profile of the two-dimensional co-culture sys...

2025-06-14 | MTBLS12599 | MetaboLights
TranscriptomicsA comparison of mono-culture and co-culture model containing fibroblasts
Organotypic three dimensional cultures of epithelial cells are grown at the air–liquid interface (ALI) and resemble the in vivo counterparts. Although the complexity of in vivo cellular responses could be better manifested in co-culture models in which additional cell-types such as fibroblasts were ...
ORGANISM(S): Homo sapiens 
2015-06-12 | E-MTAB-3462 | biostudies-arrayexpress
TranscriptomicsTranslational co-culture model reveals epithelial-mesenchymal mechanisms of lung fibrosis and identifies NF-κB as potential therapeutic target
The interaction of lung epithelial and lung mesenchymal cells was investigated in a novel co-culture model of human pulmonary fibrosis. Remarkably, co-culturing both cell types induced cell-type-specific responses, including fibroblast-to-myofibroblast differentiation and epithelial-to-mesenchymal t...
ORGANISM(S): Homo sapiens 
2022-10-03 | E-MTAB-11847 | biostudies-arrayexpress
TranscriptomicsRNA-seq time series of lung epithelial and lung mesenchymal cells in a novel co-culture model of lung fibrosis
The interaction of lung epithelial and lung mesenchymal cells (fibroblasts) was investigated in a novel co-culture model of human pulmonary fibrosis. Remarkably, co-culturing both cell types induced cell-type-specific responses, including fibroblast-to-myofibroblast differentiation and epithelial-to...
ORGANISM(S): Homo sapiens 
2022-10-03 | E-MTAB-11832 | biostudies-arrayexpress
TranscriptomicsA novel 3-D organotypic culture model by co-culturing alpha-SMA positive CAF and cholangiocarcinoma cells in a collagen matrix
The increased M-NM-1-smooth muscle-actin positive cancer-associated fibroblastic cells (CAF) in the desmoplastic stroma may relate to a more aggressive cancer and worse survival outcomes for intrahepatic cholangiocarcinoma (ICC) patients We developed a novel 3-D organotypic culture model by co-cultu...
ORGANISM(S): Rattus norvegicus 
2012-06-22 | E-GEOD-38889 | biostudies-arrayexpress
TranscriptomicsRNA-seq of Pluripotent Stem Cell-Derived Endometrial Stromal Fibroblasts in Monolayer Differentiation and in Co-culture with Endometrial Epithelial Organoids
H9 Pluripotent Stem Cells (PSC) were differentiated to Endometrial Stromal Fibroblasts (PSC-ESF) in monolayer over the course of 12 days. Gene expression was measured at day 0, day 4, day 8, and day 12 of differentiation. At day 12, PSC-ESF were dissociated from monolayer culture for co-culture with...
ORGANISM(S): Homo sapiens 
2021-05-05 | E-MTAB-10375 | biostudies-arrayexpress
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