B. pertussis mid-log phase cultures were diluted in modified Stainer-Scholte medium to a starting optical density at 600 nm of 0.03 to 0.05, then grown at 37 C with shaking until three hours beyond the log to stationary phase transition.
B. pertussis mid-log phase cultures were diluted in modified Stainer-Scholte medium to a starting optical density at 600 nm of 0.03 to 0.05, then grown at 37 C with shaking until stationary phase. Samples were taken at multiple points along the growth curve.
B. pertussis mid-log phase cultures were washed twice and resuspended at a starting OD at 600 nm of 0.05 in media containing various concentrations of glutamate.
