Ct26 - OmicsDI

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Single-Cell Extraction: For enzymatic digestion, pronase (Septomyces argeus, 1 mg/mL) was applied for 25 minutes at room temperature for P0 to P8 datasets. Cells were dissociated and triturated into a single-cell suspension in ACSF supplemented with 1% fetal calf serum (FCS) and DNase (1 µL/10 mL). Trituration was performed using three glass Pasteur pipettes of decreasing diameters. For P30 datasets, enzymatic digestion and cell dissociation were carried out using the Worthington Papain Dissociation System, following the manufacturer’s instructions. Single-Nuclei Extraction: For nuclei isolation, the Hibernate™-E Medium was removed, and 500 µL of chilled 0.1X NP40 Lysis Buffer was added. Tissue was homogenized using a Pellet Pestle (15 strokes) and incubated on ice for 5 minutes. The suspension was pipette-mixed 10 times using a wide-bore pipette tip and incubated for an additional 10 minutes on ice. Following lysis, 500 µL of chilled wash buffer was added, and the mixture was pipette-mixed 5 times using a regular-bore pipette tip. The suspension was filtered through a 30 µm cell strainer into a 50 mL tube to remove debris. The filtered suspension was transferred to a 1.5 mL tube and centrifuged at 950 × g for 10 minutes at 4°C. The supernatant was carefully removed, and the nuclei pellet was retained for downstream analysis. (2)
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Publication Date

2014 (99)
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CHEBI ID

CHEBI:162592 (1)
CHEBI:162789 (1)
CHEBI:162584 (1)
CHEBI:189968 (1)
CHEBI:195594 (1)
CHEBI:195593 (1)
CHEBI:194720 (1)
CHEBI:195590 (1)
CHEBI:195595 (1)
CHEBI:162381 (1)
CHEBI:162608 (1)
CHEBI:175878 (1)
CHEBI:163986 (1)
CHEBI:195589 (1)
CHEBI:178676 (1)
CHEBI:163801 (1)
CHEBI:193726 (1)
CHEBI:195588 (1)
CHEBI:195587 (1)
CHEBI:195586 (1)
CHEBI:73904 (1)
CHEBI:181741 (1)
CHEBI:162277 (1)
CHEBI:195585 (1)
CHEBI:163973 (1)
CHEBI:93923 (1)
CHEBI:195584 (1)
CHEBI:195583 (1)
CHEBI:183415 (1)
CHEBI:195581 (1)

Metabolite Name

Pro Pro Gly (1)
Pro Val Glu (1)
Pro Pro Asp (1)
Phenylacetohydroximoyl-glutathione (1)
Pro Ile Asn (1)
Simmondsin 2'-ferulate (1)
Gly Leu Glu (1)
Serine glutamate (1)
Gly Gly Leu (1)
D-Beta-Homophenylalanine (1)
Pro Pro Ile (1)
Cis-Entacapone (1)
Delta8-Tetrahydrocannabinol (1)
Xi-5-Dodecanolide (1)
N-octanoyl-L-Homoserine lactone (1)
N,N-Dimethyldecylamine oxide (1)
Lysine phosphoester (1)
Methyl 4-mercaptobutyrimidate (1)
Delta-Tridecanolactone (1)
Aspidocarpine (1)
Glu Leu (1)
3,4-Dimethyl-5-pentyl-2-furantridecanoic acid (1)
Pro Glu Leu (1)
Pro Glu Val (1)
Hydroxyprolyl-Methionine (1)
4'-Azidocytidine (1)
15-Octadecene-9,11,13-triynoic acid (1)
N2-Ethyl-2'-deoxyguanosine (1)
N-methylsphingosine (1)
Pro Pro Thr (1)

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2026 (50)
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2014 (10)
2019 (5)
2018 (3)
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Modelling Approach

Ordinary differential equation model (1)

Study status

Withdrawn (1)

Lab affiliation

Institute for Research in Immunology and Cancer, Department of Biochemistry, Department of Chemistry, Université de Montréal, Québec, Canada H3T 1J4 (9)
University of Vienna, Faculty of Chemistry, Department of Analytical Chemistry (3)
Professor (2)
University of Oxford (2)
Biotech Research and Innovation Centre (BRIC), Faculty of Health and Medical Sciences, University of Copenhagen (1)
Daglig leder, PROMEC Institutt for klinisk og molekylær medisin lars.hagen@ntnu.no 73598508 72573342 Laboratoriesenteret, 231.04.008, Øya, Erling Skjalgssons gate 1 PROMEC, Norway (1)
Dep. of Oncology-Pathology, Karolinska Institutet, Scilifelab, Stockholm, Sweden (1)
Jenner Institute Antigen Discovery Group University of Oxford (1)
Monash University (1)
Nanobio Measurement Group, Division of Biomedical Metrology, Korea Research Institute of Standards and Science (KRISS), Daejeon 34113, Republic of Korea (1)
Oncology Research and Development, Pfizer Inc. (1)
RyTME, Bordeaux Institute of Oncology (BRIC)-UMR1312 Inserm, B2 Ouest, Allée Geoffroy St Hilaire CS50023, 33615, Pessac, France. (1)
Sahmyook University(815, Hwarang-ro, Nowon-gu, Seoul) (1)
Shanghai Cancer Center, Fudan University,China (1)
The National Center for Nanoscience and Technology (1)
VetMedZg Laboratory of proteomics,Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia (1)
VetMedZg Laboratory,Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Institute of Biodiversity, Animal Health & Comparative Medicine, College of Medicine, Veterinary Medicine and Life Sciences, University of Glasgow, Glasgow, UK (1)

IsPrivate

False (1)

Submitter Keywords

Immuno-oncology (1)

Study type

Transcription profiling by array (425)
RNA-seq of coding RNA (101)
ChIP-seq (20)
Transcription profiling by tiling array (14)
RNA-seq of non coding RNA (14)
DNA-seq (10)
Methylation profiling by array (9)
RNA-seq of coding RNA from single cells (8)
RNA-seq of total RNA (8)
Methylation profiling by high throughput sequencing (6)
Animal - High-throughput sequencing (5)
Comparative genomic hybridization by array (5)
Other (5)
Unknown experiment type (3)
Animal - One-color microarray (3)
Cell line - High-throughput sequencing (2)
ChIP-chip by array (2)
Genotyping by array (2)
MicroRNA profiling by array (2)
ChIP-chip by tiling array (1)
One-color microarray (1)
High-throughput sequencing (1)
Bisulfite-seq (1)
RIP-seq (1)
Genotyping by high throughput sequencing (1)
Interventional (1)

Release Date

2023 (114)
2022 (112)
2014 (109)
2024 (105)
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2015 (83)
2016 (75)
2012 (66)
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2019 (62)
2013 (62)
2010 (60)
2011 (58)
2017 (47)
2008 (43)
2009 (35)
2006 (32)
2007 (26)
2026 (21)
2005 (10)
2004 (3)

Location

United States (1)

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CT26 RNA-Seq

RNA-Seq of CT26 mouse colorectal carcinoma and gene expression profiling

ORGANISM(S): Mus musculus

2014-02-07 | E-MTAB-2292 | biostudies-arrayexpress

Lentinula edodes strain:CT26

Lentinula edodes CT26 resequencing

PRJNA871497 | ENA

Mus musculus strain:CT26

Mus musculus strain:CT26 Raw sequence reads

PRJNA1132367 | ENA

Subclone of resistant CT26 murine tumor shows high sensitivity to VA7 virotherapy: A novel system to study innate immunity and antigen dominance in oncolytic immuno-virotherapy

Recently, attenuated Semliki Forest virus vector VA7 completely eliminated type I interferon (IFN) unresponsive human U87 glioma xenografts while IFN responsive mouse GL261 and CT-2A gliomas proved refractory to the oncolytic virotherapy. Here we describe in two clones of a well established Balb/c m...

ORGANISM(S): Mus musculus

2014-09-05 | E-GEOD-53001 | biostudies-arrayexpress

RNA-seq of CT26 cells expressing control or Gna13 targeting sgRNAs

CT26 cells expressing lentiviral Cas9 and sgRNAs targeting either control or Gna13

ORGANISM(S): Mus musculus

2021-10-01 | E-MTAB-10727 | biostudies-arrayexpress

RNA-seq of CT26 cells expressing TRE-GNA13 Q226L

CT26 cells expressing a lentiviral TRE-GNA13 Q226L transgene were untreated or treated with 100 ng/mL doxycycline for 48h

ORGANISM(S): Mus musculus

2021-10-01 | E-MTAB-10726 | biostudies-arrayexpress

RNA-seq of CT26 subcutaneous tumors expressing control or Gna13 targeting sgRNAs

CT26 cells expressing lentiviral Cas9 and sgRNAs targeting either control or Gna13 were transplanted into immunocompetent BALB/c mice. Tumors were harvested and processed for RNA-seq

ORGANISM(S): Mus musculus

2021-10-01 | E-MTAB-10737 | biostudies-arrayexpress

CT26 RNA-Seq

PRJEB5320 | ENA

TCR variable regions transcripts of CT26-shppp2r1a and CT26-scr samples

PRJNA781570 | ENA

Cell autonomous and non-autonomous interactions of a western-style diet and the vitamin D receptor in intestinal homeostasis and tumorigenesis

These data suggest that co-culture with macrophages increases expression of NDRG-1 in epithelial cell lines. The finding is confirmed in 2 human epithelial cell lines, and in tissue derived from mice genetically and dietetically altered to increase macrophage infiltration of the small and large int...

ORGANISM(S): Mus musculus

2014-03-12 | E-GEOD-55809 | biostudies-arrayexpress

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