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MetabolomicsThe genome analysis of Tripterygium wilfordii reveals TwCYP712K1 and TwCYP712K2 responsible for oxidation of friedelin in celastrol biosynthesis pathway

Tripterygium wilfordii is a vine used in Traditional Chinese Medicine (TCM) from the family Celastraceae. The active ingredient celastrol is a friedelane-type pentacyclic triterpenoid, with a putative role as an anti-tumor, immunosuppression, and obesity agent. Here we reported a referen...

2021-02-24 | MTBLS1080 | MetaboLights
ProteomicsLFQ of mNeonGreen-Trap IP-MS from cells expressing TFEB-mNeonGreen
Cells stably expressing TFEB-mNeonGreen proteins were grown in a 10 cm dish and treated with or without LLOMe for 1 h or 3 h, then cross-linked with 0.1% formaldehyde for 10 min at room temperature. After cross-linking was quenched with 100 mM glycine for 4 min at room temperature, cells were washed...
ORGANISM(S): Homo Sapiens (human) 
2025-08-17 | PXD043288 | JPOST Repository
ProteomicsLFQ of mNeonGreen-Trap IP-MS from cells expressing TFEB-mNeonGreen treated with MK6-83
Cells stably expressing TFEB-mNeonGreen proteins were grown in a 10 cm dish and treated with or without MK6-83 for 3 h, then cross-linked with 0.1% formaldehyde for 10 min at room temperature. After cross-linking was quenched with 100 mM glycine for 4 min at room temperature, cells were washed with ...
ORGANISM(S): Homo Sapiens (human) 
2025-08-17 | PXD053327 | JPOST Repository
ProteomicsLFQ of mNeonGreen-Trap IP-MS from cells expressing HKDC1-mNeonGreen
Cells stably expressing full-length HKDC1-mNeonGreen or △N20 HKDC1-mNeonGreen proteins were grown in a 10-cm dish and were treated with or without antimycin/oligomycin for 3h, and then cross-linked with 0.1% formaldehyde for 10min at room temperature. After cross-linking was quenched with 100mM glyc...
ORGANISM(S): Homo Sapiens (human) 
2024-01-21 | PXD040970 | JPOST Repository
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