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TranscriptomicsAn IL-1β driven neutrophil-stromal cell axis fosters a BAFF-rich microenvironment in multiple myeloma
Neutrophils are the most abundant nucleated cell type in the bone marrow. A pro-tumor bias in this cell type may have implications for bone-marrow residing malignancies, such as multiple myeloma. Here, we generated single cell transcriptomic overviews of the entire myeloid compartment, including the...
ORGANISM(S): Homo sapiens 
2023-03-31 | E-MTAB-12760 | biostudies-arrayexpress
TranscriptomicsGroup 3 innate lymphoid cells amplify YAP1-driven small intestinal repair
Ror gamma t-deficient mice lack group 3 Innate Lymphoid Cells (ILC3s) and as a result have increased tissue damage and diminished tissue repair in response to insult. To identify repair programs associated with ILC3 presence the transcriptomes of small intestinal stem cells exposed to damage in the ...
ORGANISM(S): Mus musculus 
2018-06-29 | E-MTAB-6639 | biostudies-arrayexpress
TranscriptomicsBulk RNAseq of control and 5TGM1 myeloma-bearing bone marrow T, NK, and non-hematopoietic stromal cells from C57Bl/6 mice
We hypothesized that the immune microenvironment of the bone marrow influences the progression of myeloma outgrowth in the 5TGM1 transfer model of multiple myeloma. Therefore we sorted bone marrow T, NK, and non-hematopoietic stromal cells from control and tumor-bearing C57Bl/6 mice.
ORGANISM(S): Mus musculus 
2023-02-24 | E-MTAB-12615 | biostudies-arrayexpress
TranscriptomicsscRNAseq of the bone marrow immune microenvironment in control and 5TGM1 myeloma-bearing C57Bl/6 and KaLwRij mice
We hypothesized that the immune microenvironment of the bone marrow influences the progression of myeloma outgrowth in the 5TGM1 transfer model of multiple myeloma. Therefore we sorted bone marrow T, B, NK, neutrophils, and monocytes/macrophages from control and tumor-bearing C57Bl/6 and KaLwRij mic...
ORGANISM(S): Mus musculus 
2023-02-23 | E-MTAB-12616 | biostudies-arrayexpress
TranscriptomicsDicer1 deletion in myeloid-committed progenitors causes neutrophil dysplasia and blocks macrophage/dendritic cell development in mice
MiRNAs have the potential to regulate cellular differentiation programs. However, miRNA-deficiency in primary hematopoietic stem cells (HSCs) results in HSC depletion in mice, leaving the question of whether miRNAs play a role in early-lineage decisions unanswered. To address this issue, we deleted ...
ORGANISM(S): Mus musculus 
2012-06-17 | E-GEOD-35844 | biostudies-arrayexpress
TranscriptomicsCross-tissue transcriptomic analysis of human secondary lymphoid organ residing ILC3 reveals a default quiescent state in the absence of inflammation
A cross tissue transcriptional comparison of human ILC3 isolated from non-inflamed lymph nodes (LN) and spleens, inflamed tonsils and the peripheral blood (PB). Using uniform gating and high-purity cell-sorting, ILC3 were sorted as Lineage(CD3,CD19,CD34,CD14,CD94)-CD45intCD127+CRTH2-CD117+. From LN,...
ORGANISM(S): Homo sapiens 
2017-07-31 | E-MTAB-5909 | biostudies-arrayexpress
TranscriptomicsRNA-seq of total lymph node cells from inducible ll7 gene deletion mice
Il7fx/KO Rosa26CreERT2 mice (samples 4, 5 and 6) and CreERT –ve littermate controls (samples 1, 2 and 3) were treated with tamoxifen for five days. Three weeks later, RNA was isolated from total lymph nodes of individual mice and gene expression determined by RNAseq.
ORGANISM(S): Mus musculus 
2018-02-16 | E-MTAB-6451 | biostudies-arrayexpress
TranscriptomicsRNA-seq of mouse small intestinal CCR6+ group3 innate lymphoid cells
CCR6+NKp46- ILC3 were isolated from the small intestine of untreated mice by flow cytometry. ILC3 from 3 mice were pooled for subsequent RNA sequencing.
ORGANISM(S): Mus musculus 
2019-10-11 | E-MTAB-8387 | biostudies-arrayexpress
TranscriptomicsPaired scRNAseq of the and non-hematopoietic and the immune microenvironment of aspirates from newly diagnosed Multiple Myeloma patients versus non-cancer controls
By generating a paired single cell RNA-sequencing database of the tumor niche from 10 newly diagnosed MM patients, we created a unique dataset allowing the in-depth analyses of stromal-immune interactions within the tumor microenvironment. Using this database, we identified the presence of inflammat...
ORGANISM(S): Homo sapiens 
2020-09-30 | E-MTAB-9139 | biostudies-arrayexpress
TranscriptomicsRNA sequencing of the non-hematopoietic microenvironment of aspirates from Multiple Myeloma patients at diagnosis and after treatment versus non-cancer controls
By generating a paired single cell RNA-sequencing database of the tumor niche from 10 newly diagnosed MM patients, we created a unique dataset allowing the in-depth analyses of stromal-immune interactions within the tumor microenvironment (see related accession number). Using this database, we ident...
ORGANISM(S): Homo sapiens 
2021-02-01 | E-MTAB-9285 | biostudies-arrayexpress
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