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Transformed AC16 cells were acquired from Millipore and used at P10-15 and expanded in DMEM/F-12 with 10% FBS (Gibco). Primary human ventricular cardiac fibroblasts were acquired from Promocell and expanded in FGM-3 (Promocell) and used at P3-5. For the experiment, cells were separately placed on tr...
ORGANISM(S): Homo Sapiens (human) 
Mice with transgenic expression of MYH7 with a myc-tag at the C-terminus generated in the Leinwand lab were analyzed. The transgene is expressed under control of a skeletal muscle specific MCK promoter, as previously described in: https://pmc.ncbi.nlm.nih.gov/articles/PMC11060730/ Mice were injecte...
ORGANISM(S): Mus Musculus (mouse) 
Mice were injected with two 500μL injections of 0.15M NaCl dissolved in 99.9% D2O, separated by a four-hour interval. Then, the mice were given ad libitum access to 8% D2O drinking water for the remainder of the experiment. Mice were euthanized and dissected on days 0, 1, 2, 3, 4, 6, 8, 10, 15, 20,...
ORGANISM(S): Mus Musculus (mouse) 
For calibration curve samples, human AC16 cells (Millipore) were cultured in DMEM/F12 supplemented with 10% FBS and either 6% D2O (heavy labeled population) or 6% H2O (control population) at 37°C, 5% CO2. The cells were maintained in this medium for 3 passages, each passage with a split ratio of 1:8...
ORGANISM(S): Homo Sapiens (human) 
hiPSC-CM heavy water labeling calibration standard AICS-0052-003 hiPSC (mono-allelic C-terminus mEGFP-tagged MYL7 WTC-11; Allen Institute Cell Collection) line was acquired from Coriell Institute and seeded onto Geltrex (Gibco) coated 6 well plates and maintained in mTesR Plus basal media with Supp...
ORGANISM(S): Homo Sapiens (human) 
Fractional synthesis calibration cells for hiPSC AICS-0052-003 hiPSC (mono-allelic C-terminus mEGFP-tagged MYL7 WTC-11; Allen Institute Cell Collection) line was acquired from Coriell Institute and seeded onto Geltrex (Gibco) coated 6 well plates and maintained in mTesR Plus basal media with Supplem...
ORGANISM(S): Homo Sapiens (human) 
To examine the protein spatial and temporal changes upon carfilzomib-mediated proteasome inhibition in cardiac cells, we produced human iPSC-derived cardiomyocytes using a standard small molecule based protocol. Cardiomyocyte identity was confirmed by morphology, observation of contraction, and the ...
ORGANISM(S): Homo Sapiens (human) 
The functionality of proteins is dependent on their spatial and temporal distributions, neither of which is directly measured by static protein abundance. Here we report a mass spectrometry-based proteomics workflow and data analysis pipeline, named Simultaneous Proteome Localization and Turnover (...
ORGANISM(S): Homo Sapiens (human) 
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