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MetabolomicsIsotope Labeled Reactivity-Based Metabolomics Reveal Cysteine as a Potent In Vivo Scavenger of Lactoylglutathione

Metabolite adducts are increasingly being recognized as important intermediates in cellular metabolism and as circulating messengers. Methylglyoxal (MG) is a highly reactive metabolite involved in diabetes and aging, via formation of a wide variety of protein adducts. Though new types of protein ...

2025-04-29 | MTBLS6287 | MetaboLights
MetabolomicsProteomics and tracer metabolomics link GAPDH ISGylation to glycolytic control

Ubiquitin-like protein ISG15 (interferon-stimulated gene 15) has been implicated in the regulation of central carbon metabolism, but conflicting findings across experimental systems have limited mechanistic insight. Here, we applied a multi-omics approach in cells ectopically expressing the ISGyl...

2026-03-02 | MTBLS12619 | MetaboLights
ProteomicsSupporting information for tryptic EPO glycopeptide LC-MS analysis via cysteine aminoethylation
Supplementary information for a technical note describing the application of cysteine aminoethylation for the site-specific glycosylation analysis of recombinant human EPO using trypsin as sole protease.
ORGANISM(S): Cho Cells 
2020-04-21 | MSV000085318 | MassIVE
ProteomicsChemoselective umpolung of thiols to episulfoniums for cysteine bioconjugation
Modification of native cysteine is an important tool in protein research and relies on fast and chemoselective reactions under bioambient conditions. Nucleophilic cysteine can be functionalized with electrophiles, such as maleimides, that carry specific chemical groups, depending on the desired appl...
ORGANISM(S): Escherichia Coli 
2023-12-20 | PXD038723 | JPOST Repository
ProteomicsE2/E3-independent ubiquitin-like protein conjugation by Urm1 is directly coupled to cysteine persulfidation
Posttranslational modifications by ubiquitin-like proteins (UBL) are essential for nearly all cellular processes. Ubiquitin related modifier 1 (Urm1) is a non-canonical UBL which plays a key role in tRNA anticodon thiolation as a sulfur carrier protein (SCP). While Urm1 has also been observed to con...
ORGANISM(S): Chaetomium Thermophilum (ncbitaxon:209285) Saccharomyces Cerevisiae (ncbitaxon:4932) Homo Sapiens (ncbitaxon:9606) 
2021-11-16 | MSV000088390 | MassIVE
ProteomicsExtending the coverage of Lys-C/trypsin-based bottom-up proteomics using cysteine S-aminoethylation
In this study, S-aminoethylation of Cys (AE-Cys) was carried out with 2-bromoethylamine to form pseudo-Lys, which was then digested with Lys-C or trypsin to improve proteome coverage in bottom-up proteomics. A model study with bovine serum albumin showed that the C-terminal side of Cys was successfu...
ORGANISM(S): Homo Sapiens (human) Bos Taurus 
2023-09-27 | PXD045433 | JPOST Repository
ProteomicsCysteine oxidation as a regulatory mechanism of Arabidopsis plastidial NAD-dependent malate dehydrogenase
Malate dehydrogenases (MDHs) catalyze a reversible NAD(P)-dependent-oxidoreductase reaction that plays an important role in central metabolism and redox homeostasis of plant cells. Recent studies suggest a moonlighting function of plastidial NAD-dependent MDH (plNAD-MDH) in plastid biogenesis, indep...
ORGANISM(S): Escherichia Coli 
2024-04-29 | PXD048384 | JPOST Repository
ProteomicsIntegrated Dissection of Cysteine Oxidative Modification Proteome During Cardiac Hypertrophy
Cysteine oxidative modification of cellular proteins is crucial for many aspects of cardiac hypertrophy development. However, integrated dissection of multiple types of cysteine oxidation proteome in cardiac hypertrophy is currently missing. Here we developed a novel discovery platform encompassing ...
ORGANISM(S): Mus musculus (Mouse) 
2018-08-29 | PXD010336 | Pride
Mouse Cardiac hypertrophy Heart Biotin switch Dimethyl labeling Cysteine oxidation Reversible cysteine oxidation Cysteine sulfinylation Cysteine sulfonylation Quantitative proteomics 
ProteomicsKetone Bodies-mediated Cysteine Modifications Discovered by Chemical Proteomics
All the studies of ketone body-dependent post-translational modifications (PTMs), notably those mediated by ketone bodies, β-hydroxybutyrate (Bhb) and acetoacetate (Acac), have focused on lysine acylations. However, given the chemically diverse and reactive nature of metabolites generated, it remain...
ORGANISM(S): Homo Sapiens 
PXD066371 |
ProteomicsRegulation of hyperoxia-induced neonatal lung injury via posttranslational cysteine redox modifications
Lung tissue from normoxia- or hyperoxia-treated control or SCNN1B overexpression mice were processed with a redox proteomics workflow and analyzed by LC-MS/MS with TMT based quantification. Data was searched with MS-GF+ using PNNL's DMS Processing pipeline.
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2022-08-18 | MSV000090157 | MassIVE
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