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GenomicsEndogenous DAF-16 genome-wide recruitment under low Insulin signaling condition in Caenorhabditis elegans
In order to understand the complexity of gene regulation downstream of IIS, we used anti-DAF-16 antibody, we report the first genome-wide ChIP-sequencing study of endogenous DAF-16 recruitment in daf-2(e1370). We also report the average bin-wise normalized read count of ZFP-1 and DAF-16 on DAF-16 s...
ORGANISM(S): Caenorhabditis elegans 
2016-03-01 | E-GEOD-63865 | biostudies-arrayexpress
ProteomicsAnalysis of C. elegans DAF-16 phosphorylation in different genetic backgrounds.
C. elegans DAF-16/FOXO phosphorylation in different genetic backgrounds was analyzed by mass spectrometry of affinity purified HA-tagged DAF-16. Genetic backgrounds studied include par-1 hypomorphic mutants, PAR-1 knockdown using an auxin-inducible degron (AID), daf-2(e1370) and akt-1(ok525) mutants...
ORGANISM(S): Caenorhabditis elegans 
2025-12-29 | PXD053590 | Pride
Foxo Par-1 Daf-16 Phosphorylation Daf-2 Mark Akt-1 
TranscriptomicsEndogenous DAF-16 genome-wide recruitment under low Insulin signalling condition in Caenorhabditis elegans
In order to understand the complexity of gene regulation downstream of IIS, we did RNA-seq in mixed culture in wild-type, daf-2(e1370), daf-16(mgDf50);daf-2(e1370) and daf-2(e1370);daf-12(m20 and correlated it with ChIP-seq data RNA-seq profile of different mutants in mix stage
ORGANISM(S): Caenorhabditis elegans 
2016-03-01 | E-GEOD-67975 | biostudies-arrayexpress
TranscriptomicsDAF-16/FoxO and EGL-27/GATA promote developmental growth in response to persistent somatic DNA damage [N2, daf-2, daf-16, daf-2;daf-16]
Genome maintenance defects cause complex disease phenotypes characterized by developmental failure, cancer susceptibility and premature aging. It remains poorly understood how DNA damage responses function during organismal development and maintain tissue functionality when DNA damage accumulates wi...
ORGANISM(S): Caenorhabditis elegans 
2014-11-18 | E-GEOD-51162 | biostudies-arrayexpress
ProteomicsTurboID-mediated proximity labeling of DAF-16/FOXO in wild-type and daf-2(e1370) mutants in C. elegans
TurboID-mediated proximity labeling of the aging and stress regulatory transcription factor DAF-16/FOXO in wild-type and daf-2(e1370) mutants in C. elegans. Endogenous DAF-16 was C-terminally tagged with the biotin ligase TurboID using CRISPR/Cas9- genome editing. In vivo proximity labeling of the ...
ORGANISM(S): Caenorhabditis elegans 
2025-12-29 | PXD053591 | Pride
Foxo Turboid Insulin / igf signaling Daf-16 Caenorhabditis elegans Proximity labeling 
TranscriptomicsRNA sequencing analysis of DAF-16 target gene expression when math-33 function is genetically inactivated
In this study we have investigated the effect of loss of math-33 activity on DAF-16-mediated target gene regulation in C. elegans under conditions of reduced Insulin/IGF-1 signaling (IIS). Using whole nematode RNA sequencing experiments we found that the daf-2(e1370)-mediated induction and repressio...
ORGANISM(S): Caenorhabditis elegans 
2016-06-22 | E-GEOD-70117 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of long-lived C.elegans daf-2 pathway mutants with wild type/short-lived mutants
Comparison of long-lived daf-2 pathway mutants with wild type/short-lived mutants. A: age-1 fer-15; fem-1 vs. fer-15; fem-1. B: age-1 fer-15; fem-1 vs. fer-15; fem-1. C: fer-15; daf-2(mu150); fem-1 vs. fer-15; fem-1. D: fer-15; daf-2(mu150); fem-1 vs. fer-15; fem-1. E: daf-16::gfp in daf...
ORGANISM(S): Caenorhabditis elegans 
2003-07-17 | E-MEXP-7 | biostudies-arrayexpress
TranscriptomicsRNAi knock down of daf-2, daf-3, daf-16 in C. elegans over 0-48 hours
Comparison of sterile worms grown on Control Vector, daf-2 RNAi, and daf-2 + daf-16 RNAi. Synchronized fer-15(b26); fem-1(hc17) animals were grown on RNAi bacteria at 25ºC, induced with IPTG on Day 1 of adulthood, and collected from 0-48 hours (10 time points) and 0-196 hours (10 time points); RNAi ...
ORGANISM(S): Caenorhabditis elegans 
2003-07-17 | E-MEXP-8 | biostudies-arrayexpress
GenomicsmRNA-seq analysis of Caenorhabditis elegans wild type N2, daf-16(mu86), daf-18(ok480) and daf-16(mu86) daf-18(ok480) starved L1 larvae
mRNA-seq analysis of Caenorhabditis elegans wild type N2, daf-16(mu86), daf-18(ok480) and daf-16(mu86) daf-18(ok480) starved L1 larvae
PRJNA1182399 | ENA
TranscriptomicsRNAi knock down of daf-16 in C. elegans over 0-192 hours
Comparison of sterile worms grown on Control Vector, daf-2 RNAi, and daf-2 + daf-16 RNAi. Synchronized fer-15(b26); fem-1(hc17) animals were grown on RNAi bacteria at 25ºC, induced with IPTG on Day 1 of adulthood, and collected from 0-48 hours (10 time points) and 0-196 hours (10 time points); RNAi ...
ORGANISM(S): Caenorhabditis elegans 
2003-07-17 | E-MEXP-9 | biostudies-arrayexpress
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