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ProteomicsDARPin F10 interactome with AP-MS
Identifying the HDAC6 targeting DARPin F10 interacting protein in human cells. Proteins are expressed as GFP fused protein and captured by GFP-trap beads. DARPin CTR is a control DARPin that binds to nothing.
ORGANISM(S): Homo sapiens (Human) 
2023-10-17 | PXD031799 | Pride
Human Hdac6 Darpin 
TranscriptomicsTransient GFP-fused DARPin expression effects on transcription
GFP-tagged DARPin F10 and CTR are expressed in A549 cells, then GFP positive cells are sorted by GFP positive with FACS. Cells are pelleted and total RNA is extracted. The aim is to check DARPin F10 effects on gene expression.
ORGANISM(S): Homo sapiens 
2022-03-12 | E-MTAB-11496 | biostudies-arrayexpress
ProteomicsUbiquitination site analysis of eGFP by DARPin-based bioPROTACs analysed by LC-MS-MS
We investigated design principles of DARPin-based bioPROTACs targeting the model substrate GS-eGFP (eGFP with an n-terminal tag, necessary for proteasomal unfolding). Using a highly active E3 ligase domain from CHIP and nine well-characterized DARPins, we evaluated their ability to induce proteasoma...
ORGANISM(S): Homo sapiens (Human) 
2025-06-05 | PXD063877 | Pride
Ubiquitination Darpin Bioprotac Egfp 
GenomicsTransient GFP-fused DARPin expression effects on transcription
Transient GFP-fused DARPin expression effects on transcription
PRJEB51294 | ENA
TranscriptomicsRNA-seq of DARPin F10 expressing cells as compared to Wt A549
De novo synthesized protein DARPin F10 can target HDAC6 ZnF domain, and inhibits Influenza A virus and ZIKA virus infection. Our data shows it interferes with the virus uncoating step. To exclude the possibility that F10 upregulates interferon pathways, we checked the transcription of the cell
ORGANISM(S): Homo sapiens 
2021-11-07 | E-MTAB-11101 | biostudies-arrayexpress
TranscriptomicsRNAseq of HeLa and U2OS cells treated with either Nutlin, Darpin C10 targeting p53 or a control DARPin
p53 expression and transcriptional activity is repressed in human papillomavirus positive cells by the expression of viral E6 protein. E6 forms a complex with cellular E6AP protein which can lead to ubiquitination of p53, thus repressing its tumor-suppressive capabilities. In this experiment, the po...
ORGANISM(S): Homo sapiens 
2025-09-05 | E-MTAB-13602 | biostudies-arrayexpress
GenomicsRNAseq of HeLa and U2OS cells treated with either Nutlin, Darpin C10 targeting p53 or a control DARPin
RNAseq of HeLa and U2OS cells treated with either Nutlin, Darpin C10 targeting p53 or a control DARPin
PRJEB70860 | ENA
GenomicsRNA-seq of DARPin F10 expressing cells as compared to Wt A549
RNA-seq of DARPin F10 expressing cells as compared to Wt A549
PRJEB48421 | ENA
ProteomicsProtein mass spectrometry raw data of M7A, M7GA, M7A-DC, and M7GA-DC for DAR calculation in study of MSLN-directed DARPin-drug conjugates
Mesothelin (MSLN) is an attractive therapeutic target for precision cancer treatments. Here, we identify a MSLN-targeting DARPin, M7, which specifically binds to the protease-sensitive C-terminal region of MSLN. Furthermore, we develop two auristatin-based DARPin-drug conjugates (DARPin-DCs), M7A-DC...
ORGANISM(S): Escherichia coli 
2025-09-05 | PXD065630 | Pride
Lc/ms Protein-drug conjugates 
UnknownBeyond Antibodies: The DARPin® Drug Platform.
Not available
S-EPMC7312107 | biostudies-literature
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