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ProteomicsHistidine Kinase Inhibitors Impair Shoot Regeneration in Arabidopsis thaliana via Cytokinin Signalling and SAM Patterning Determinants
Reversible protein phosphorylation is a post-translational modification involved in virtually all plant processes, as it mediates protein activity and signal transduction. Here, we probe dynamic protein phosphorylation during de novo shoot organogenesis in Arabidopsis thaliana. We find that applicat...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-09-28 | PXD030754 | Pride
Cytokinin signalling Phosphoproteomics Kinase inhibitors Regeneration Organogenesis 
ProteomicsFunctional annotation of proteins for signaling network inference in non-model species
Molecular biology aims to understand the molecular basis of cellular responses, unravel dynamic regulatory networks, and model complex biological systems. However, these studies remain challenging in non-model species as a result of poor functional annotation of regulatory proteins, like kinases or ...
ORGANISM(S): Glycine max 
2023-06-13 | PXD037601 | Pride
Phosphoproteomics Protein family Soybean Temperature 
ProteomicsTemperature-induced changes in wheat leaf and flower phosphoproteome
Wheat (Triticum ssp.) is one of the most important human food sources as well as livestock feed, but is very sensitive to changes in temperature. Specifically, processes during leaf growth and photosynthesis and during flower and seed development are affected by high temperature. While this has been...
ORGANISM(S): Triticum aestivum (Wheat) 
2018-10-19 | PXD008703 | Pride
Triticum aestivum Heat Phosphoproteome Wheat 
ProteomicsProteome-wide cellular thermal shift assay reveals novel crosstalk between brassinosteroid and auxin signaling
We demonstrated that bikinin changed the thermal stability of some of its target proteins as well as several known or putative interacting proteins of Arabidopsis GSK3s. By combining the thermal- and the phospho-proteomes of bikinin we uncovered the auxin carrier PIN-FORMED1 (PIN1) as a novel substr...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-03-08 | PXD029936 | Pride
Brassinosteroids Chemical genetics Auxin，ms/ms spectra Arabidopsis cell suspension 
ProteomicsAuxin-triggered changes in the Arabidopsis root tip (phospho)proteome reveal novel root growth regulators
Canonical auxin signalling starts with auxin binding to the receptor complex, followed by modulation of gene transcription and protein abundance (Tan et al., 2007; Chapman and Estelle, 2009; Slade et al., 2017). However, recent studies also showed an alternative mechanism in roots involving intra-ce...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2021-09-09 | PXD021267 | Pride
Auxin Phosphoproteomoics Plant 
ProteomicsComparative (phospho)proteomic profiling of wheat shoots from different cultivars
Wheat is a cereal grain and one of the world’s major food crops. Recent advances in wheat genome sequencing are by now facilitating genomic and proteomic analyses of this crop. However, little is known about the protein levels of hexaploid versus tetraploid wheat cultivars, and knowledge on phosphor...
ORGANISM(S): Triticum aestivum (Wheat) Triticum turgidum subsp. durum 
2017-04-03 | PXD005437 | Pride
Proteomics Plant Phosphoproteomics Wheat 
ProteomicsA sequential MAP kinase cascade regulates early mechanical signaling
Plants respond to mechanical stimulations such as wind, rain, touching or wounding to safeguard their development and survival. Rapid activation of Mitogen Activated Protein Kinases (MAPKs) by mechanical stimuli was already reported nearly 25 years ago, but the mechanism and function of this phenome...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-06-16 | PXD060728 | Pride
Arabidopsis Touching Mitogen activated protein kinases Wounding 
ProteomicsEarly mannitol-triggered changes in the Arabidopsis leaf (phospho)proteome
Drought is one of the most detrimental environmental stresses to which plants are exposed. Especially mild drought is relevant to agriculture and significantly affects plant growth and development. In plant research, mannitol is often used to mimic drought stress and study the resulting responses. I...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-10-19 | PXD008900 | Pride
Arabidopsis thaliana Mild osmotic stress Signalling Phosphoproteome 
ProteomicsA conserved signaling complex controls warm temperature-mediated plant growth
The model plant Arabidopsis thaliana responds to mild high temperature by increased elongation growth of organs to enhance cooling capacity, in a process called thermomorphogenesis. Our understanding of the genetic regulation of thermomorphogenesis has increased in recent years. However, hardly anyt...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2021-03-25 | PXD015468 | Pride
Arabidopsis Phosphorylation High temperature 
ProteomicsA conserved signaling complex controls warm temperature-mediated plant growth
The model plant Arabidopsis thaliana responds to mild high temperature by increased elongation growth of organs to enhance cooling capacity, in a process called thermomorphogenesis. Our understanding of the genetic regulation of thermomorphogenesis has increased in recent years. However, hardly anyt...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2021-03-25 | PXD015483 | Pride
Thermomorphogenesis Arabidopsis Kinase 
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