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GenomicsAnalyses of Rad2, Mediator and RNA Polymerase II in different yeast mutant context.
We performed ChIP-seq analyses of Rad2, Mediator (Med17 and Med5) and RNA Polymerase II in Kin28-ts16 mutant, Med17-Q444P and Med17-Q444P/M442L mutants and in an Rpb9 deleted strain.
ORGANISM(S): Saccharomyces cerevisiae S288C 
2019-07-01 | E-MTAB-7081 | biostudies-arrayexpress
TranscriptomicsMicrofluidic platform for monitoring Saccharomyces cerevisiae mutation accumulation assay
Saccharomyces cerevisiae wildtype (YPH) and ung1-deleted strains were cultivated in mutation accumulation experiments over several bottlenecks (0-50-100-150). Two different cutlure systems were used either (i) using random colony selection and plating on petri dish (classical); or (ii) a microfluidi...
ORGANISM(S): Saccharomyces cerevisiae S288C 
2021-08-01 | E-MTAB-10259 | biostudies-arrayexpress
GenomicsFunctional interplay between Mediator and RSC chromatin remodeling complex in nucleosome-depleted region maintenance at promoters
ChIPseq of Mediator and RSC in wildtype or mutant context reveal their co-occupancy genome-wide. In addition MNAse-seq experiments in the same contexts shows the specific impact of the mutations on nucleosome positioning/remodelling.
ORGANISM(S): Saccharomyces cerevisiae S288C 
2023-05-11 | E-MTAB-12198 | biostudies-arrayexpress
GenomicsGenome-wide distribution of Rad26 and Rad1-Rad10 in relation to Mediator and RNA polymerase II
ChIPseq of Rad26, Rad1 and Rad10 proteins indicate their presence on chromatin in absence of UV-stress. In addition, in kin28ts mutant context, their genomic profiles change suggesting the influence of both Mediator complex and PolII transcription
ORGANISM(S): Saccharomyces cerevisiae S288C 
2022-06-17 | E-MTAB-10828 | biostudies-arrayexpress
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