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ProteomicsProteomic Analysis of the Human Amniotic Mesenchymal Stromal Cell Secretome by integrated approaches via Filter-Aided Sample Preparation
LC-MS proteomic analysis using top-down and bottom-up combined strategies via Filter-Aided Sample Preparation (FASP) was applied to the secretome of mesenchymal stromal cells isolated from the amniotic membrane of human term placenta in the attempt to define the profile of its intact as well as dige...
ORGANISM(S): Homo sapiens (Human) 
2024-10-25 | PXD055623 | Pride
Filter-aided sample preparation Proteomics Human amniotic mesenchymal stromal cells Secretome 
ProteomicsAnalysis of the GFP-labelled β-dystroglycan interactome in Hek-293 transfected cells reveals novel intracellular networks
So far very little is known about the fine regulation of some of these intracellular interactions of β-DG and how they are perturbed in diseases. To start filling this gap, HEK-293 cells were transiently transfected with a plasmid carrying the β-DG subunit with GFP fused at its C-terminus. Immunopre...
ORGANISM(S): Homo sapiens (Human) 
2024-02-12 | PXD043909 | Pride
Nuclear envelopathies Muscular dystrophy Lamina-associated polypeptide-1b (lap1b) Mass spectrometry proteomics Dystroglycan Torsin 1a interacting protein 1. 
ProteomicsCharacterization of N-terminal acetylation of α-hemoglobin stabilizing protein (AHSP) by top-down high resolution mass spectrometry of oral fluids from human preterm newborns.
The project reports the identification in oral fluid samples of preterm newborns of AHSP (Alpha-hemoglobin Stabilizing Protein), an erythroid-specific protein forming stable complexes with free α-hemoglobin preventing its harmful aggregation and pro-oxidant activity during red blood cell development...
ORGANISM(S): Homo sapiens (Human) 
2025-07-18 | PXD061305 | Pride
Oral fluid top-down proteomics  preterm newborn biomarkers  alpha-hemoglobin stabilizing protein (ahsp)  lc-ms  neonatal hematological disorders 
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