C2C12 (ATCC, #CRL-1772), a myoblast cell line from the C3H mouse strain, was grown in DMEM (Dulbecco’s Modified Eagle’s Medium, Gibco), supplemented with 10% fetal bovine serum (FBS) (Euroclone, Pero, Italy) and 2 mM L-glutamine (Gibco), and maintained in culture at 37 °C with 5% CO₂, without reachi...
ORGANISM(S): Mus musculus
