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UnknownEndogenous Mobilization of Bone-Marrow Cells Into the Murine Retina Induces Fusion-Mediated Reprogramming of Muller Glia Cells.
Müller glial cells (MGCs) represent the most plastic cell type found in the retina. Following injury, zebrafish and avian MGCs can efficiently re-enter the cell cycle, proliferate and generate new functional neurons. The regenerative potential of mammalian MGCs, however, is very limited. Here, we sh...
S-EPMC5952225 | biostudies-literature
UnknownFunctional Rescue of Dopaminergic Neuron Loss in Parkinson's Disease Mice After Transplantation of Hematopoietic Stem and Progenitor Cells.
Parkinson's disease is a common neurodegenerative disorder, which is due to the loss of dopaminergic neurons in the substantia nigra pars compacta (SNpc) and for which no definitive cure is currently available. Cellular functions in mouse and human tissues can be restored after fusion of bone marrow...
S-EPMC4919540 | biostudies-literature
UnknownThe Chemokine Receptors Ccr5 and Cxcr6 Enhance Migration of Mesenchymal Stem Cells into the Degenerating Retina.
Cell therapy approaches hold great potential for treating retinopathies, which are currently incurable. This study addresses the problem of inadequate migration and integration of transplanted cells into the host retina. To this end, we have identified the chemokines that were most upregulated durin...
S-EPMC7854301 | biostudies-literature
UnknownZinc-finger-based transcriptional repression of rhodopsin in a model of dominant retinitis pigmentosa.
Despite the recent success of gene-based complementation approaches for genetic recessive traits, the development of therapeutic strategies for gain-of-function mutations poses great challenges. General therapeutic principles to correct these genetic defects mostly rely on post-transcriptional gene ...
S-EPMC3085076 | biostudies-literature
UnknownMyosin7a deficiency results in reduced retinal activity which is improved by gene therapy.
Mutations in MYO7A cause autosomal recessive Usher syndrome type IB (USH1B), one of the most frequent conditions that combine severe congenital hearing impairment and retinitis pigmentosa. A promising therapeutic strategy for retinitis pigmentosa is gene therapy, however its pre-clinical development...
S-EPMC3753344 | biostudies-literature
UnknownWnt/β-catenin signaling pathway safeguards epigenetic stability and homeostasis of mouse embryonic stem cells.
Mouse embryonic stem cells (mESCs) are pluripotent and can differentiate into cells belonging to the three germ layers of the embryo. However, mESC pluripotency and genome stability can be compromised in prolonged in vitro culture conditions. Several factors control mESC pluripotency, including Wnt/...
S-EPMC6353868 | biostudies-literature
UnknownSerotype-dependent packaging of large genes in adeno-associated viral vectors results in effective gene delivery in mice.
Vectors derived from adeno-associated virus (AAV) are promising for human gene therapy, including treatment for retinal blindness. One major limitation of AAVs as vectors is that AAV cargo capacity has been considered to be restricted to 4.7 kb. Here we demonstrate that vectors with an AAV5 capsid (...
S-EPMC2298836 | biostudies-literature
UnknownThe Wnt-dependent master regulator NKX1-2 controls mouse pre-implantation development.
Embryo size, specification, and homeostasis are regulated by a complex gene regulatory and signaling network. Here we used gene expression signatures of Wnt-activated mouse embryonic stem cell (mESC) clones to reverse engineer an mESC regulatory network. We identify NKX1-2 as a novel master regulato...
S-EPMC11103935 | biostudies-literature
UnknownAAV-mediated photoreceptor transduction of the pig cone-enriched retina.
Recent success in clinical trials supports the use of adeno-associated viral (AAV) vectors for gene therapy of retinal diseases caused by defects in the retinal pigment epithelium (RPE). In contrast, evidence of the efficacy of AAV-mediated gene transfer to retinal photoreceptors, the major site of ...
S-EPMC3131697 | biostudies-literature
Unknownβ-Catenin safeguards the ground state of mousepluripotency by strengthening the robustness of the transcriptional apparatus.
Mouse embryonic stem cells cultured with MEK (mitogen-activated protein kinase kinase) and GSK3 (glycogen synthase kinase 3) inhibitors (2i) more closely resemble the inner cell mass of preimplantation blastocysts than those cultured with SL [serum/leukemia inhibitory factor (LIF)]. The transcriptio...
S-EPMC7439582 | biostudies-literature
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