Pdzd8-3xHA knock-in mice and control littermates at postnatal 10 days were put to sleep using medetomidine hydrochloride, midazolam and butorphanol. Pups were then put on the ice for 5 minutes and exsanguinated by terminal intracardial perfusion with ice-cold 2% paraformaldehyde in PBS. The neocorte...
ORGANISM(S): Mus Musculus (mouse)