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ProteomicsDesign and evaluation of a cyclobutane diazirine alkyne tag for photoaffinity labeling in cells
Alkyl diazirines are frequently used in photoaffinity labeling to trap and identify small molecule–protein interactions. How-ever, the alkyl diazirine can preferentially label acidic amino acids and acidic protein surfaces in a pH-dependent manner, presumably via a long-lived alkyl diazo intermediat...
ORGANISM(S): Homo sapiens (Human) 
2024-01-04 | PXD035954 | Pride
Cereblon Diazirine Thalidomide Photoaffinity labeling Binding site mapping 
ProteomicsDeciphering Diazirine Reaction Mechanism: through Irradiation Modulation for Residue-Specific Distance Restraints in Protein Photo-Crosslinking
The diazirine photoreactive group has been widely used for photo-labeling and photo-cross-linking (PXL) of proteins. Yet, due to the mechanistic complexity of diazirine photo-reaction, site-specific and quantitative analysis of protein PXL data remains challenging. Herein, we have built an in-line p...
ORGANISM(S): Bos taurus (Bovine) 
2024-06-22 | PXD048452 | Pride
Photo cross-linking Multiple reaction monitoring Optical power density Distance restraints Alkyl diazirine 
ProteomicsEvaluation of fully functionalized diazirine tags for chemical proteomic applications
The use of photo-affinity labeling (PAL) reagents for the mapping of noncovalent small molecule-protein interactions has become widespread. Recently, several ‘fully-functionalized’ (FF) chemical tags have been developed wherein a photoactivatable capture group, an enrichment handle, and a functional...
ORGANISM(S): Homo sapiens (Human) 
2021-05-18 | PXD025652 | Pride
Fully-functionlalized Diazirine Photoaffinity 
TranscriptomicsRNA-Seq of A375 cells treated with tetracycline-based crosslinking probes, with and without competition by parent tetracyclines, to show specific binding of the tetracycline analogs Col-3 and doxycycline on rRNA substructures of the human ribosome
Upon finding that the tetracyclines COL-3 and doxycycline target unique rRNA substructures of the 80S ribosome (E-MTAB-7147), we confirmed these putative ribosomal binding sites by showing that tetracycline-based crosslinking probes are specifically competed from these rRNA structures by their paren...
ORGANISM(S): Homo sapiens 
2018-08-31 | E-MTAB-7161 | biostudies-arrayexpress
UnknownA diazirine-based photoaffinity etoposide probe for labeling topoisomerase II.
Not available
S-EPMC2818565 | biostudies-literature
UnknownMetabolism of diazirine-modified N-acetylmannosamine analogues to photo-cross-linking sialosides.
Not available
S-EPMC3178686 | biostudies-literature
UnknownValidation of Trifluoromethylphenyl Diazirine Cholesterol Analogues As Cholesterol Mimetics and Photolabeling Reagents.
Not available
S-EPMC8740335 | biostudies-literature
UnknownEfficient Cross-Linking through C-H Bond Insertion of Unfunctionalized Commodity Materials Using Diazirine-Containing Polymers.
Not available
S-EPMC11580386 | biostudies-literature
UnknownDesign and Evaluation of a Cyclobutane Diazirine Alkyne Tag for Photoaffinity Labeling in Cells.
Not available
S-EPMC11647570 | biostudies-literature
UnknownReducing Limitation in Probe Design: The Development of a Diazirine-Compatible Suzuki-Miyaura Cross Coupling Reaction.
Not available
S-EPMC6331167 | biostudies-literature
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