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TranscriptomicsPBMC response to B. pertussis filamentous hemagglutinin stimulation in vitro
Filamentous hemagglutinin (FHA), Bordetella pertussis' key adhesin, is both present at the cell surface and released in the extracellular milieu. To comprehend the role free FHA plays in the pathogenic process, we analyzed the global transcriptional changes in human peripheral blood mononuclear cell...
ORGANISM(S): Homo sapiens 
2008-10-29 | E-GEOD-8802 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Bordetella pertussis from mid-log phase cultures diluted to two concentrations and grown beyond the log to stationary phase transition to determine growth phase-associated patterns of transcript abundance
B. pertussis mid-log phase cultures were diluted in modified Stainer-Scholte medium to a starting optical density at 600 nm of 0.03 to 0.05, then grown at 37 C with shaking until three hours beyond the log to stationary phase transition.
ORGANISM(S): Bordetella parapertussis 
2006-12-31 | E-TABM-100 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Bordetella pertussis from mid-log phase cultures diluted to two concentrations and sampled at multiple points along the growth curve to determine growth phase-associated patterns of transcript abundance
B. pertussis mid-log phase cultures were diluted in modified Stainer-Scholte medium to a starting optical density at 600 nm of 0.03 to 0.05, then grown at 37 C with shaking until stationary phase. Samples were taken at multiple points along the growth curve.
ORGANISM(S): Bordetella pertussis 
2006-12-31 | E-TABM-101 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Bordetella pertussis strains in response to glutamate availablility
B. pertussis mid-log phase cultures were washed twice and resuspended at a starting OD at 600 nm of 0.05 in media containing various concentrations of glutamate.
ORGANISM(S): Bordetella parapertussis 
2006-12-04 | E-TABM-99 | biostudies-arrayexpress
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