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ChIP-seq for H3K27me3 and Ring1B was performed in WT mESCs and mESCs containing catalytically inactive Ring1B (I53A mutant). Cells expressing catalytically inactive Ring1B maintain the spatial distribution of Ring1B and H3K27me3 but at reduced levels. These findings support the notion that PRC2 recr...
ORGANISM(S): Mus musculus 
This experiment was designed to determine the extent of gene misregulation in mESCs containing catalytically dead Ring1B in comparison to mESCs lacking Ring1B. Polyadenylated mRNA was prepared from wildtype mESCs (WT), mESCs deficient for Ring1B (KO) and mESCs cells containing catalytically dead Rin...
ORGANISM(S): Mus musculus 
The goal of this study is to understand the genome-wide alterations in chromatin landscape induced by a histone mutation (H3.3K36M) derived from chondroblastomas Examination of genome-wide Ring1b distribution in cells expressing H3.3WT and H3.3K36M.
ORGANISM(S): Mus musculus 
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