Cells were grown to ~50% confluency before RNA polymerase activity was blocked with 10 µg/mL Actinomycin D (Sigma) in DMSO. Control cells were treated with DMSO alone. Transcriptional inhibition of N2A cells was conducted for 32 h with cells harvested at time zero (0 h) and after 30 min, 2, 4, 8, 16...
ORGANISM(S): Mus musculus