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TranscriptomicsMicroarray analysis of V. cholerae genes differentially expressed in rabbit ileal loop fluid and mucus
This SuperSeries is composed of the following subset Series: GSE6217: Microarray analysis of V. cholerae genes differentially expressed in 12 h rabbit ileal loop fluid GSE24405: Microarray analysis of V. cholerae genes differentially expressed in 8 h rabbit ileal loop mucus GSE24406: Microarray anal...
ORGANISM(S): Vibrio cholerae 
2010-09-28 | E-GEOD-24408 | biostudies-arrayexpress
TranscriptomicsMicroarray analysis of V. cholerae genes differentially expressed in 12 h rabbit ileal loop mucus
The mucus and cell-associated bacteria were isolated as a single fraction from ileal loops twelve hours post inoculation by scraping the epithelial surface of the intestine with a disposable plastic cell scraper after the loops had been cut open and gently rinsed in PBS buffer to wash away remaining...
ORGANISM(S): Vibrio cholerae 
2010-09-28 | E-GEOD-24406 | biostudies-arrayexpress
TranscriptomicsMicroarray analysis of V. cholerae genes differentially expressed in 8 h rabbit ileal loop mucus
The mucus and cell-associated bacteria were isolated as a single fraction from ileal loops eight hours post inoculation by scraping the epithelial surface of the intestine with a disposable plastic cell scraper after the loops had been cut open and gently rinsed in PBS buffer to wash away remaining ...
ORGANISM(S): Vibrio cholerae 
2010-09-28 | E-GEOD-24405 | biostudies-arrayexpress
TranscriptomicsMicroarray analysis of V. cholerae genes differentially expressed in 8h rabbit ileal loop fluid.
Transcriptional analysis of wild-type V. cholerae isolated from the rabbit ileal loop was performed by quickly pelleting bacteria from the fluid accumulated in the ileal loops after 8 h of infection. The bacteria were then resuspended in Trizol reagent and frozen on dry ice. The wild-type bacteria f...
ORGANISM(S): Vibrio cholerae 
2010-09-28 | E-GEOD-24407 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Vibro cholerae from rabbit ileal loop fluid 12 h post infection to identify differentially expressed genes
Transcriptional analysis of wild-type V. cholerae isolated from the rabbit ileal loop was performed by quickly pelleting bacteria from the fluid accumulated in the ileal loops after 12 h of infection. The bacteria were then resuspended in Trizol reagent and frozen on dry ice. The wild-type bacteria ...
ORGANISM(S): Vibrio cholerae 
2006-11-06 | E-SMDB-3832 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Vibrio cholerae wild type, hapR and rpoS deletions in mid-exponential or stationary phase concluding that rpoS controls the Vibrio cholerae mucosal escape response
For expression analysis of wild-type V. cholerae, hapR, and rpoS deletion mutants in mid-exponential or stationary phase, the strains were grown to either OD600 of 0.3 or for 11 h in LB media at 37 0C, and bacteria from 2-ml culture were quickly pelleted, resuspended in Trizol reagent (GIBCO/BRL, Sa...
ORGANISM(S): Vibrio cholerae 
2006-11-06 | E-SMDB-3833 | biostudies-arrayexpress
TranscriptomicsDNA microarray analysis of genes positively regulated by TfoX
Vibrio cholerae strains A1552tfoX/pBAD and A1552tfoX/pBAD-tfoX were diluted 1:100 from over night cultures and grown in LB liquid medium containing 0.2 % L-arabinose and 100 μg/ml ampicillin. At OD600=0.3, samples were quickly harvested in a microcentrifuge at room temperature and the bacterial pel...
ORGANISM(S): Vibrio cholerae 
2010-06-30 | E-GEOD-3577 | biostudies-arrayexpress
TranscriptomicsDNA microarray analysis of genes positively regulated by HapR
Vibrio cholerae strains A1552 and ATN140 (A1552-delta-hapR) were diluted 1:100 from over night cultures and grown aerobically (at 250 rpm) in M9 minimal medium supplemented with vitamins (MEM vitamin solution, BRL) and 0.5% lactate. At OD600 ~ 0.3 2 mM (GlcNAc)6 (Seikagaku America, East Falmouth, MA...
ORGANISM(S): Vibrio cholerae 
2010-06-30 | E-GEOD-3576 | biostudies-arrayexpress
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