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Transcriptomicsm1A marks translation initiation sites in human and mouse messenger RNA [microarray]
We developed a novel approach, m1A-seq, for high-resolution mapping of the transcriptome-wide m1A landscape, based on antibody-mediated capture followed by massively parallel sequencing. Using this method we performed immunodepletion of methylated transcipts to assess the average methylation level I...
ORGANISM(S): Homo sapiens 
2016-02-12 | E-GEOD-76057 | biostudies-arrayexpress
Transcriptomicsm1A marks translation initiation sites in human and mouse messenger RNA [Sequencing]
We developed a novel approach, m1A-seq, for high-resolution mapping of the transcriptome-wide m1A landscape, based on antibody-mediated capture followed by massively parallel sequencing Identification of m1A modified sequences in human, mouse and yeast cell lines
ORGANISM(S): Homo sapiens 
2016-02-10 | E-GEOD-70485 | biostudies-arrayexpress
Transcriptomicsm6A mapping in human RNA (untreated)
We developed a novel approach, m6A-seq, for high-resolution mapping of the transcriptome-wide m6A landscape, based on antibody-mediated capture followed by massively parallel sequencing. Identification of m6A modified sequences in HepG2 cells.
ORGANISM(S): Homo sapiens 
2012-04-25 | E-GEOD-37003 | biostudies-arrayexpress
TranscriptomicsMETTL3 KD in HepG2 cells
To gain insight into possible processes that require m6A for their function, METTL3 was knocked down (KD) in HepG2 cells by siRNA transfections Differential expression analysis of METTL3 KD versus mock-transfected HepG2 cells, in 2 biological replicates
ORGANISM(S): Homo sapiens 
2012-04-25 | E-GEOD-37001 | biostudies-arrayexpress
Transcriptomicsm6A mapping in human RNA (with treatments)
We developed a novel approach, m6A-seq, for high-resolution mapping of the transcriptome-wide m6A landscape, based on antibody-mediated capture followed by massively parallel sequencing. Identification of m6A modified sequences in HepG2 cells. HepG2 cells were incubated with either IFNg (200ng/ml) o...
ORGANISM(S): Homo sapiens 
2012-04-25 | E-GEOD-37002 | biostudies-arrayexpress
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