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GenomicsTotal RNA Pol2 ChIP-seq in mES cells treated with flavopiridol (Cdk9 inhibitor) after DSIF and NELF knockdown
Recent studies have found that promoter-proximal pausing occurs at most Pol2-regulated genes. DSIF and NELF function as negative elongation factors and promote Pol2 pausing. P-TEFb, whose enzymatic activity lies in the kinase Cdk9, positively regulates the transition into productive elongation by ...
ORGANISM(S): Mus musculus 
2011-01-19 | E-GEOD-26658 | biostudies-arrayexpress
GenomicsGenome-wide map of RNA polymerase II, NELF, and DSIF in HeLa cells
Most metazoan promoters have RNA polymerase II (Pol II) paused slightly downstream of the transcription start site by NELF and DSIF. This pausing keeps these promoters available for rapid induction by P-TEFb, whose activity causes NELF to dissociate and Pol II and DSIF to elongate on the gene. ChIP-...
ORGANISM(S): Homo sapiens 
2014-02-01 | E-GEOD-53008 | biostudies-arrayexpress
TranscriptomicsThe DSIF subunits Spt4 and Spt5 have several unexpected, distinct roles in regulating immunoglobulin class switch recombination
Class-switch recombination (CSR), induced by activation-induced cytidine deaminase, can be divided into two phases: DNA cleavage of the switch (S) regions, and the joining of the cleaved ends of the different S regions. Here, we show that the DSIF complex (Spt4 and Spt5), a transcription elongation ...
ORGANISM(S): Mus musculus 
2013-10-22 | E-GEOD-33206 | biostudies-arrayexpress
TranscriptomicsDSIF and RNA polymerase II CTD Phosphorylation Coordinate the Recruitment of Rpd3S to Actively Transcribed Genes
Histone deacetylase Rpd3 is part of two distinct complexes: the large (Rpd3L) and small (Rpd3S) complexes. While Rpd3L targets specific promoters for gene repression, Rpd3S is recruited to ORFs to deacetylate histones in the wake of RNA polymerase II, to prevent cryptic initiation within genes. Meth...
ORGANISM(S): Saccharomyces cerevisiae 
2010-11-28 | E-GEOD-22636 | biostudies-arrayexpress
UnknownStructure of paused transcription complex Pol II-DSIF-NELF.
Not available
S-EPMC6245578 | biostudies-literature
UnknownDSIF modulates RNA polymerase II occupancy according to template G + C content.
Not available
S-EPMC9326580 | biostudies-literature
GenomicsG+C content of transcribed sequence modulates DSIF-assisted DNA occupancy by RNA polymerase II [RNA-seq]
G+C content of transcribed sequence modulates DSIF-assisted DNA occupancy by RNA polymerase II [RNA-seq]
PRJNA716749 | ENA
GenomicsG+C content of transcribed sequence modulates DSIF-assisted DNA occupancy by RNA polymerase II [ChIP-seq]
G+C content of transcribed sequence modulates DSIF-assisted DNA occupancy by RNA polymerase II [ChIP-seq]
PRJNA716629 | ENA
UnknownCTCF regulates NELF, DSIF and P-TEFb recruitment during transcription.
Not available
S-EPMC4802788 | biostudies-literature
GenomicsGenome-wide map of RNA polymerase II, NELF, and DSIF in HeLa cells
Most metazoan promoters have RNA polymerase II (Pol II) paused slightly downstream of the transcription start site by NELF and DSIF. This pausing keeps these promoters available for rapid induction by P-TEFb, whose activity causes NELF to dissociate and Pol II and DSIF to elongate on the gene. ChIP-...
ORGANISM(S): Homo sapiens 
2014-02-01 | GSE53008 | GEO
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