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TranscriptomicsTriticum urartu strain:PI 428198 Genome sequencing and Transcriptome or Gene expression
For this project, we have sequenced, assembled and annotated a transcriptome of a diploid wheat Triticum urartu accession PI 428198. The sequencing libraries were prepared from shoot and root tissues harvested from 2-3 week old seedlings. All sequencing was carried out on the Illumina HiSeq platform...
ORGANISM(S): Triticum urartu 
2013-06-11 | E-MTAB-5037 | biostudies-arrayexpress
TranscriptomicsSmall RNAs in transgenic TaNAM-RNAi wheat plants
To explore the effect of stable RNAi on the small RNA (sRNA) population in wheat, we constructed a sRNA library from hexaploid wheat that expresses an RNAi construct under the 35S promoter that targets the endogenous NO APICAL MERISTEM (TaNAM) gene. The presence of this RNAi transgene causes a 40% r...
ORGANISM(S): Triticum aestivum 
2010-05-28 | E-GEOD-22048 | biostudies-arrayexpress
TranscriptomicsEffect of downregulation of the high grain protein content (GPC) gene on the wheat transcriptome during monocarpic senescence
When GPC transcription factor in hexaploid wheat is down-regulated by stable RNA interference (GPC RNAi), senescence is significantly delayed and grain protein content together with the overall nutrient partitioning to the grain is greatly reduced. mRNA-seq was used to catalogue the genes that are r...
ORGANISM(S): Triticum aestivum 
2011-09-23 | E-GEOD-25759 | biostudies-arrayexpress
TranscriptomicsRNA-seq studies using wheat PHYTOCHROME B and PHYTOCHROME C mutants reveal shared and specific functions in the regulation of flowering and shade-avoidance pathways
The phytochromes (PHYs) are a family of photoreceptors which absorb light of red and far-red wavelengths to modulate developmental responses in photosynthetic organisms. We developed knockout mutants of PHYB and PHYC in wheat and performed replicated RNA-seq studies to analyze and compare the regulo...
ORGANISM(S): Triticum turgidum subsp. durum 
2016-06-29 | E-GEOD-79049 | biostudies-arrayexpress
TranscriptomicsRNA sequencing of stripe rust infected wheat leaves and isolated haustoria
The fungus Puccinia striiformis f.sp. tritici (PST) is the causal pathogen of stripe rust in wheat. New highly virulent PST races appeared at the beginning of this century and spread rapidly causing significant yield losses in wheat production worldwide. Race PST-08/21 was isolated in the UK in 2008...
ORGANISM(S): Puccinia striiformis f. sp. tritici 08/21 
2013-08-19 | E-GEOD-42496 | biostudies-arrayexpress
TranscriptomicsRegulation of Zn and Fe transporters by the GPC1 gene during wheat monocarpic senescence
TaGPC1 and TaGPC2 are NAC-domain transcription factors which accelerate the onset of senescence and facilitate nutrient translocation in wheat. We developed knockout mutants of these genes in tetraploid wheat and used RNA-seq to identify the effect of these mutations on the wheat flag leaf transcrip...
ORGANISM(S): Triticum turgidum subsp. durum 
2014-12-23 | E-GEOD-60635 | biostudies-arrayexpress
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