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TranscriptomicsTranscription profiling of mouse metaphase II oocytes collected from 5-6 week old mice with those collected from 40-42 week old mice
Decreasing oocyte competence with maternal aging is a major factor in human infertility. To investigate the age-dependent molecular changes in a mouse model, we compared the expression profiles of metaphase II oocytes collected from 5-6 week old mice with those collected from 40-42 week old mice usi...
ORGANISM(S): Mus musculus 
2004-09-01 | E-MEXP-150 | biostudies-arrayexpress
TranscriptomicsTranscript copy number estimation using a mouse whole-genome oligonucleotide microarray (22k Linearity)
E12.5 mouse whole embryo and E12.5 placenta total RNA were pooled to create 25:75, 50:50, and 75:25 ratio mixtures, based on Bioanalyzer quantitation. These samples, along with the original unmixed RNAs, were used as templates for duplicate linear amplification labeling reactions. cRNA target mixtu...
ORGANISM(S): Mus musculus 
2010-06-09 | E-GEOD-3508 | biostudies-arrayexpress
TranscriptomicsTranscript copy number estimation using a mouse whole-genome oligonucleotide microarray (WGA 2.1 Spike Test)
E12.5 mouse whole embryo, E12.5 placenta, embryonic stem (ES) cells, and trophoblast stem (TS) cells were compared on a novel microarray design to validate the system. The in situ-synthesized 60-mer oligonucleotide microarray platform contains approximately 44,000 DNA features, and was designed to ...
ORGANISM(S): Mus musculus 
2005-10-28 | E-GEOD-3509 | biostudies-arrayexpress
TranscriptomicsGenome-wide transcriptome analyses by the RNA-seq method
We performed the whole transcriptome analysis in Zscan4 positive ES cells (Em+) and Zscan4 negative ES cells (Em-) by using FACS-sorted MC1-ZE7 ES cells. Whole RNA-seq in Zscan4 positive and negative cells
ORGANISM(S): Mus musculus 
2015-09-10 | E-GEOD-58619 | biostudies-arrayexpress
TranscriptomicsAge-associated alteration of gene expression patterns in mouse oocytes
Decreasing oocyte competence with maternal aging is a major factor in human infertility. To investigate the age-dependent molecular changes in a mouse model, we compared the expression profiles of metaphase II oocytes collected from 5-6 week old mice with those collected from 40-42 week old mice us...
ORGANISM(S): Mus musculus 
2010-06-06 | E-GEOD-1646 | biostudies-arrayexpress
TranscriptomicsIn situ-synthesized novel microarray optimized for mouse stem cell and early developmental expression profiling: Part2
E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples and compared on a novel microarray design to validate the system. A "self-versus-self" experimental design was u...
ORGANISM(S): Mus musculus 
2010-06-30 | E-GEOD-3513 | biostudies-arrayexpress
TranscriptomicsIn situ-synthesized novel microarray optimized for mouse stem cell and early developmental expression profiling: Part1
E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples and compared on a novel microarray design to validate the system. A "dye-swapped" experimental design was used t...
ORGANISM(S): Mus musculus 
2010-06-30 | E-GEOD-3516 | biostudies-arrayexpress
TranscriptomicsIn situ-synthesized novel microarray optimized for mouse stem cell and early developmental expression profiling: Part 3
E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples at various input levels. A "full-scale" reference was prepared using the standard input level of 6.0 ug total RN...
ORGANISM(S): Mus musculus 
2010-06-30 | E-GEOD-3507 | biostudies-arrayexpress
TranscriptomicsNovel RNA-binding activity of MYF5 enhances Ccnd1/Cyclin D1 mRNA translation during myogenesis
Skeletal muscle contains long multinucleated and contractile structures known as muscle fibers, which arise from the fusion of myoblasts into nucleated myotubes during myogenesis. The myogenic regulatory factor (MRF) MYF5 is the earliest to be expressed during myogenesis and functions as a transcri...
ORGANISM(S): Mus musculus 
2016-08-08 | E-GEOD-73704 | biostudies-arrayexpress
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