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TranscriptomicsTranscription profiling of Bos taurus immature oocytes and 8-cell embryos to assess the impact of the loss of sample representation caused by the use of global linear amplification (T7-IVT) during microarray hybridization sample preparation
Assessing the impact of the loss of sample representation caused by the use of global linear amplification (T7-IVT) during microarray hybridization sample preparation.
ORGANISM(S): Bos taurus 
2009-03-25 | E-MEXP-2099 | biostudies-arrayexpress
TranscriptomicsCSF2 x gender interactions on the transcriptome of Day 15 bovine extra-embryonic membrane
We aimed to study the effects of CSF2 treatment on the transcriptome of the Day 15 female and male embryo. Within the raw files, channel 1= Cy3 and channel 2= Cy5 Transcriptional profiling of bovine extra-embryonic membrane at Day 15 post-insemination produced by IVF. Oocytes were matured and ferti...
ORGANISM(S): Bos taurus 
2014-10-01 | E-GEOD-55364 | biostudies-arrayexpress
TranscriptomicsEffect of ovarian stimulation on oocyte gene expression in cow
The objective of the study was to analyze the impact of FSH on transcriptome changes of in vivo bovine oocytes. Oocytes were collected from naturally ovulated and superovulated animals at 2 hours pre-LH surge, 6 hours post-LH surge, and 22 hours post-LH surge. Six-condition experiment. Biological re...
ORGANISM(S): Bos taurus 
2011-07-31 | E-GEOD-27696 | biostudies-arrayexpress
TranscriptomicsAnalysis of the gene expression pattern of bovine blastocysts at three stages of development
Blastocyst formation is a primordial event of pre-implantation development since it is required for pregnancy establishment and progression. The blastocyst plays a pivotal role because it is the stage at which the embryo is transferred and starts coordinated cross-talks with the mother. It is also t...
ORGANISM(S): Bos taurus 
2011-02-17 | E-GEOD-25606 | biostudies-arrayexpress
TranscriptomicsTrancriptomic analysis of PKA and PKC signalling gene cascade in cultured human ovarian granulosa cells (KGN) [FSK treatments]
The oocyte’s capacity to complete maturation, to succeed fertilization and to reach the blastocyst stage is what defines the oocyte’s competence. The oocyte acquires this competence working closely with somatic cells of the follicle. Cumulus and granulosa cells provided support for the oocyte’...
ORGANISM(S): Homo sapiens 
2016-07-20 | E-GEOD-84541 | biostudies-arrayexpress
TranscriptomicsTrancriptomic analysis of PKA and PKC signalling gene cascade in cultured human ovarian granulosa cells (KGN) [PMA treatments]
The oocyte’s capacity to complete maturation, to succeed fertilization and to reach the blastocyst stage is what defines the oocyte’s competence. The oocyte acquires this competence working closely with somatic cells of the follicle. Cumulus and granulosa cells provided support for the oocyte’...
ORGANISM(S): Homo sapiens 
2016-07-20 | E-GEOD-84542 | biostudies-arrayexpress
TranscriptomicsHybridization of human oocyte cDNA library on the multi-species oocyte array to assess for gene conservation using a temperature stringency criteria
Human oocyte cDNA library was hybridized on a multi-species oocyte array (Bovine, Mouse, Frog) Temperature stringency criteria was used to evaluate the conservation degree of oocyte genes among vertebrates (Bovine, Mouse, Frog) 2 technical replicates on distinct array were made at each pre-determine...
ORGANISM(S): Homo sapiens 
2010-12-15 | E-GEOD-23963 | biostudies-arrayexpress
TranscriptomicsRapidly cleaving bovine embryos have a better developmental potential and a distinctive mRNA pattern
Transcriptome analysis of in vitro-produced bovine 2-cell embryos according to their time to the first cleavage. Three time points were established in order to collect all the 2-cell embryos present at each moment to contrast only the two exteme embryo subpopulations: fast against slow. 4 biological...
ORGANISM(S): Bos taurus 
2013-09-14 | E-GEOD-50633 | biostudies-arrayexpress
TranscriptomicsEffect of vitrification using the Cryotop method on the gene expression profile of in vitro-produced bovine embryos
Changes in gene expression induced by the Cryotop vitrification procedure in bovine blastocysts using Agilent EmbryoGENE microarray slides. Bovine in vitro-produced embryos at the blastocyst stage (144 to 156 hours post insemination) were vitrified using the Cryotop system and compared with non-vitr...
ORGANISM(S): Bos indicus 
2015-08-03 | E-GEOD-65953 | biostudies-arrayexpress
TranscriptomicsBovine cumulus cells transcriptome in relation with oocyte chromatin configuration of the germinal vesicle
Gene expression analysis in cc from COCs with different chromatin configuration (GV0-3) Bovine cumulus cells (4 biological replicates) were collected and grouped according to the oocyte different chromatin configuration of the germinal vesicle described by Lodde et al. 2007. Four GV stages correspon...
ORGANISM(S): Bos taurus 
2016-04-04 | E-GEOD-79886 | biostudies-arrayexpress
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